Morphology and anatomy of leaf, stem and petiole of Luvunga crassifolia tanaka (rutaceae)

Shunmugam, Sugathini and Rosli, Nur Syamilah and Manickam, Sugumaran and Mohd Yusoff, Nur Fatiha and Alitheen, Noorjahan Banu and Namasivayam, Parameswari (2021) Morphology and anatomy of leaf, stem and petiole of Luvunga crassifolia tanaka (rutaceae). Malaysian Journal of Fundamental and Applied Sciences, 17 (6). pp. 818-828. ISSN 2289-5981,

Full text not available from this repository.
Official URL:


Luvunga crassifolia is an underutilized plant in the Citrus family. Other than brief morphological descriptions, there are no published reports on other identification features of this plant. Thus, the current study was aimed to investigate macroscopic and microscopic diagnostic features of L. crassifolia leaves, stems, and petioles. Macroscopic characterization, optimization of histological procedure, and histochemical analyses of differential stains were carried out on the leaves, stems, and petioles of L. crassifolia. The histological method was optimized by modifying a few parameters including the number of fixation days, dehydration duration with degraded series of butanol, clearing and infiltration durations. Embedding and sectioning were performed on the tissues after infiltration. Histochemical analyses were carried out using differential stains to identify the cellular components in leaf, stem and, petiole tissue sections. This study showed that L. crassifolia leaves are amphistomatic. The compound leaves are trifoliate and arranged alternately. Pellucid dots were observed on both adaxial and abaxial leaf surfaces. Leaf cross-section consisted of schizogenous secretory cavities, whereas stem and petiole cross-sections show abundant schizolysigenous secretory cavities. Xylem, phloem, and pericyclic fibers were also found in the leaf, stem, and petiole sections. Calcium oxalates were present in the leaf and stem sections, while non-glandular trichomes were detected on stem and petiole sections. The information obtained from this study will be helpful for the identification and future taxonomic-related studies of this plant species. © Copyright Shunmugam et al.

Item Type: Article
Funders: UPM High Impact Putra Grant[GPB/2017/9559900], Graduate Research Fellowship (GRF) from UPM
Uncontrolled Keywords: Calcium oxalates;Morphology;Pellucid dots;Secretory cavities
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QD Chemistry
Q Science > QK Botany
Divisions: Faculty of Science
Depositing User: Ms Zaharah Ramly
Date Deposited: 02 Nov 2022 06:26
Last Modified: 02 Nov 2022 06:26

Actions (login required)

View Item View Item