Thymoquinone enhances sperm DNA integrity in nicotineinduced infertile male rats

Rosli, Farah Dayana and Hashim, Noor Hashida and Yusuf, Yusmin Mohd and Osman, Khairul and Ibrahim, Siti Fatimah and Kabir, Nurul (2019) Thymoquinone enhances sperm DNA integrity in nicotineinduced infertile male rats. Tropical Journal of Pharmaceutical Research, 18 (6). pp. 1219-1225. ISSN 1596-5996, DOI

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To assess the effects of thymoquinone (TQ) on the integrity of sperm DNA in nicotineinduced sperm impairment in rats. Methods: Adult male Sprague Dawley rats were randomized into four equal groups: Control group received normal saline orally for 60 days; nicotine group was subcutaneously injected with 5 mg/kg/day nicotine for 30 days and then given normal saline for the next 30 days; TQ group was given normal saline for 30 days followed by TQ at 5 mg/kg/day for 30 days; and nicotine-TQ group received 5 mg/kg of nicotine for 30 days and 5mg/kg of TQ for another 30 days. Sperm DNA breakages were evaluated using Comet assay. The expression levels of protamine 1 (PT1) and transition nuclear protein 2 (Tnp2) genes which are essential for the proper compaction of the sperm DNA were analyzed by quantitative polymerase chain reaction (qPCR). Results: Thymoquinone significantly decreased DNA fragmentation in the sperm of nicotine-treated rats. However, there was no change in PT1 gene expression. Tnp2 was downregulated in the nicotine group and slightly upregulated in nicotine-TQ group (p < 0.05). Conclusion: The results demonstrate the potential benefits of TQ in improving sperm DNA quality of nicotine-induced male infertility. © 2021 University of Benin. All rights reserved.

Item Type: Article
Funders: University Malaya Research Grant[UMRG-RG378/15AFR]
Uncontrolled Keywords: Thymoquinone;Infertility;Nicotine;Sperm;DNA integrity;Protamine 1;Transition nuclear protein 2
Subjects: Q Science > QD Chemistry
R Medicine
R Medicine > RM Therapeutics. Pharmacology
Divisions: Centre for Foundation Studies in Science
Depositing User: Ms Zaharah Ramly
Date Deposited: 28 Oct 2022 04:09
Last Modified: 28 Oct 2022 04:09

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