Ultra performance liquid chromatography (mini-review)

Basharat, Rabia and Kotra, Vijay and Loong, Lean Yen and Mathews, Allan and Kanakal, Mahibub Mahamadsa and Dev, Ch B. Praveena and Nyamathulla, Shaik and Varala, Ravi and Ming, Long Chiau and Rao, Krs Sambasiva and Babu, B. Hari and Alam, M. Mujahid (2021) Ultra performance liquid chromatography (mini-review). Oriental Journal of Chemistry, 37 (4). pp. 847-857. ISSN 0970-020X, DOI https://doi.org/10.13005/ojc/370411.

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Chromatography is a widely used analytical tool for separating a mixture of compounds into individual component. High performance liquid chromatography (HPLC) is one of the most important methods used for the separation, identification and quantification of a compounds present in a mixture. It meets many criteria of analysis but its main drawbacks are it is relatively time consuming to run a chromatogram and consumes high amount of solvent compared to other analytical methods. There is a need to develop a method which can overcome these drawbacks of HPLC. Ultra performance liquid chromatography (UPLC) is the new approach which opens novel direction in the field of liquid chromatography. It works on similar principle but shows better performance than conventional HPLC. UPLC is a technique of liquid chromatography with improved runtime and sensitivity with less than 2 mu m particle size. The UPLC separation process is carried out under very high pressure (up to100 MPa). Additionally, it reduces the cost of reagent with shorter run time as compared to conventional HPLC. This article updated until 2020, provides a general review on the principle, instrumentation and application of UPLC in different fields of science.

Item Type: Article
Uncontrolled Keywords: Ultra performance liquid chromatography; Separation; Quantification; Resolution; Sensitivity; Instrumentation and applications
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Q Science > QP Physiology
Divisions: Faculty of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Ms Zaharah Ramly
Date Deposited: 07 Sep 2022 01:08
Last Modified: 07 Sep 2022 01:08
URI: http://eprints.um.edu.my/id/eprint/35115

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