Lignosus rhinocerus (cooke) ryvarden: a medicinal mushroom that stimulates neurite outgrowth in pc-12 cells

Eik, L.F. and Naidu, M. and David, P. and Wong, K.H. and Tan, Y.S. and Sabaratnam, V. (2012) Lignosus rhinocerus (cooke) ryvarden: a medicinal mushroom that stimulates neurite outgrowth in pc-12 cells. Evidence-Based Complementary and Alternative Medicine, 2012. p. 320308. ISSN 1741-427X, DOI PMID: 22203867.


Download (1MB)
Official URL:


A national treasure mushroom, Lignosus rhinocerus, has been used to treat variety of ailments by local and indigenous communities in Malaysia. The aim of this study was to investigate the potential of the most valuable part of L. rhinocerus, the sclerotium, on neurite outgrowth activity by using PC-12Adh cell line. Differentiated cells with one thin extension at least double the length of the cell diameter were scored positive. Our results showed that aqueous sclerotium L. rhinocerus extract induced neurite outgrowths of 24.4% and 42.1% at 20 μg/mL (w/v) of aqueous extract alone and a combination of 20 μg/mL (w/v) aqueous extract and 30 ng/mL (w/v) of NGF, respectively. Combination of NGF and sclerotium extract had additive effects and enhanced neurite outgrowth. Neuronal differentiation was demonstrated by indirect immunofluorescence of neurofilament protein. Aqueous sclerotium extract contained neuroactive compounds that stimulated neurite outgrowth in vitro. To our knowledge this is the first report on neurite-stimulating activities of L. rhinocerus.

Item Type: Article
Additional Information: Mushroom Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia -----> Correspondence should be addressed to Vikineswary Sabaratnam,
Uncontrolled Keywords: Mushroom
Subjects: R Medicine
Divisions: Faculty of Medicine
Faculty of Science > Institute of Biological Sciences
Depositing User: Mr. Faizal Hamzah
Date Deposited: 04 Jul 2012 01:51
Last Modified: 29 Jan 2020 06:03

Actions (login required)

View Item View Item