Characterisation of PALB2 tumours through whole-exome and whole-transcriptomic analyses

Ng, Pei Sze and Pan, Jia Wern and Ahmad Zabidi, Muhammad Mamduh and Rajadurai, Pathmanathan and Yip, Cheng Har and Reuda, Oscar M. and Dunning, Alison M. and Antoniou, Antonis C. and Easton, Douglas F. and Caldas, Carlos and Chin, Suet-Feung and Teo, Soo Hwang (2021) Characterisation of PALB2 tumours through whole-exome and whole-transcriptomic analyses. NPJ Breast Cancer, 7 (1). ISSN 2374-4677, DOI

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Rare protein-truncating variants (PTVs) in PALB2 confer increased risk to breast cancer, but relatively few studies have reported the characteristics of tumours with PALB2 PTVs. In this study, we describe molecular characteristics of tumours with either germline or somatic alterations in PALB2. DNA from fresh frozen tumour tissues and matched peripheral blood lymphocytes for 560 breast cancer patients was subjected for whole-exome sequencing (WES), and RNA from tumour tissues was subjected to RNA sequencing (RNA-seq). We found six cases with germline and three with somatic protein-truncating variants in PALB2. The characteristics of tumours in patients with PALB2 PTVs were similar to those with BRCA1 and BRCA2 PTVs, having significantly more somatic alterations, and a high proportion of the mutational signature and genomic scar scores characteristic of deficiencies in homologous recombination (HR), compared to tumours arising in non-carriers. Unlike tumours arising in patients with BRCA1 and BRCA2 PTVs, PALB2 tumours did not have high prevalence of TP53 somatic alterations or an enriched immune microenvironment. In summary, PALB2 tumours show the homologous recombination deficiencies characteristic of BRCA1 and BRCA2 tumours, and highlight the potential clinical relevance of PALB2 mutational status in guiding therapeutic choices.

Item Type: Article
Funders: Newton-Ungku Omar Fund (MRC)[MR/P012442/1], Wellcome Trust European Commission[v203477/Z/16/Z], European Commission[634935], Cancer Research UK[A16942], Scientex Foundation
Uncontrolled Keywords: Breast-cancer;Mutational processes;Copy number;Reveals;Repair
Subjects: R Medicine
R Medicine > RD Surgery
Divisions: Faculty of Medicine
Depositing User: Ms Zaharah Ramly
Date Deposited: 30 May 2022 04:15
Last Modified: 30 May 2022 04:15

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