Culturable bacteria in adults of a Southeast Asian black fly, Simulium tani (Diptera:Simuliidae)

Lee, Hai-Yen and Loong, Shih Keng and Ya`cob, Zubaidah and Low, Van Lun and Teoh, Boon Teong and Ahmad-Nasrah, Siti Noraisah and Yap, Phui Chyng and Sofian-Azirun, Mohd and Takaoka, Hiroyuki and AbuBakar, Sazaly and Adler, Peter H. (2021) Culturable bacteria in adults of a Southeast Asian black fly, Simulium tani (Diptera:Simuliidae). Acta Tropica, 219. ISSN 0001-706X, DOI

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Although the microbiome of blood-feeding insects serves an integral role in host physiology, both beneficial and pathogenic, little is known of the microbial community of black flies. An investigation, therefore, was undertaken to identify culturable bacteria from one of Malaysia's most common black flies, Simulium tani Takaoka and Davies, using 16S rDNA sequencing, and then evaluate the isolates for antibiotic resistance and virulence genes. A total of 20 isolates representing 11 bacterial species in four genera were found. Five isolates showed beta-hemolysis on Columbia agar, and virulence genes were found in three of these isolates. Some degree of resistance to six of the 12 tested antibiotics was found among the isolates. The baseline data from this study suggest rich opportunities for comparative studies exploring the diversity and roles of the microbiome of S. tani and other Southeast Asian black flies.

Item Type: Article
Funders: Ministry of Higher Education, Malaysia under the Higher Institution Centre of Excellence (HICoE) program[MO002-2019]
Uncontrolled Keywords: Antibiotic resistance;Bacillus;Microbiome;Virulence genes; Vector;Infecious-diseases
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms Zaharah Ramly
Date Deposited: 14 Jun 2022 07:33
Last Modified: 14 Jun 2022 07:33

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