Facile synthesis of biocompatible sub-5 nm alginate-stabilised gold nanoparticles with sonosensitising properties

Beishenaliev, Adilet and Faruqu, Farid Nazer and Leo, Bey Fen and Lit, Lei Cheng and Loke, Yean Leng and Chang, Chia-Ching and Teo, Yin Yin and Chik, Zamri and Foo, Yiing Yee and Chung, Lip Yong and Kiew, Lik Voon (2021) Facile synthesis of biocompatible sub-5 nm alginate-stabilised gold nanoparticles with sonosensitising properties. Colloids and Surfaces A-Physicochemical and Engineering Aspects, 627. ISSN 0927-7757, DOI https://doi.org/10.1016/j.colsurfa.2021.127141.

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The use of ultrasmall gold nanoparticles (usAuNPs, diameters <5 nm) in cancer diagnosis and therapy has been increasing due to their unique tumour tissue/cell penetrating traits and renal-excretable properties. In order to produce usAuNPs with narrow polydispersity, toxic stabilising agents were normally coated on usAuNP surface during their synthesis. This has necessitated post-synthesis surface modification to minimise stabilising-agentrelated toxicity in order to qualify usAuNPs for in vivo use. Nevertheless, the average sizes of usAuNPs that were surface-modified using the reported methods were often found to be above the renal filtration threshold (5 nm), hence limiting their renal excretion and suitability for in vivo use. Here, we report a novel and facile synthesis of biocompatible and renal-clearable usAuNPs using sodium alginate as the stabilising agent. The alginate-stabilised usAuNPs (usAuNPs(ALG)) had an average size of 4.5 +/- 0.2 nm with narrow polydispersity (0.21), a zeta potential of -30.4 mV, and were stable in water for up to 45 days. They were haemocompatible and non-toxic in vitro at concentrations up to 100 mu g/ml (4T1 breast cancer cells). Further, usAuNPs(ALG) were found to generate reactive oxygen species and exert dose-dependent sonotoxicity to 4T1 and MDA-MB-231 cells in vitro at a level equivalent to that of the larger AuNPs (>5 nm), but upon ultrasound irradiation at 2-4-fold lower frequency (1 MHz) and intensity (0.5 W/cm(2)) than those previously reported in AuNP-mediated SDT. This suggests the potential use of the usAuNPs(ALG) as sonosensitising agents for anti-cancer adjunctive therapy. Overall, this study reported the novel method for the synthesis of biocompatible usAuNPs with considerable sonosensitising properties and the potential of these sub-5 nm nanoparticles for in vivo applications.

Item Type: Article
Funders: Human Life Advancement Foundation, Malaysia, 2019 Southeast Universitiy Joint Research Scheme (SATU JRS)[UM9], 2019 Southeast Universitiy Joint Research Scheme (SATU JRS)[ST031-2019]
Uncontrolled Keywords: Gold nanoparticles (AuNPs);Sodium alginate; Biocompatibility;Sonodynamic therapy (SDT);Reactive oxygen species
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms Zaharah Ramly
Date Deposited: 15 Jun 2022 04:06
Last Modified: 15 Jun 2022 04:06
URI: http://eprints.um.edu.my/id/eprint/34421

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