Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin

Tarantini, Francesco Saverio and Brunati, Mara and Taravella, Anna and Carrano, Lucia and Parenti, Francesco and Hong, Kar Wai and Williams, Paul and Chan, Kok Gan and Heeb, Stephan and Chan, Weng C. (2021) Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin. PLoS ONE, 16 (11). ISSN 1932-6203, DOI

Full text not available from this repository.


As part of a screening programme for antibiotic-producing bacteria, a novel Actinomadura species was discovered from a soil sample collected in Santorini, Greece. Preliminary 16S rRNA gene sequence comparisons highlighted Actinomadura macra as the most similar characterised species. However, whole-genome sequencing revealed an average nucleotide identity (ANI) value of 89% with A. macra, the highest among related species. Further phenotypic and chemotaxonomic analyses confirmed that the isolate represents a previously uncharacterised species in the genus Actinomadura, for which the name Actinomadura graeca sp. nov. is proposed (type strain 32-07(T)). The G+C content of A. graeca 32-07 is 72.36%. The cell wall contains DL-diaminopimelic acid, intracellular sugars are glucose, ribose and galactose, the predominant menaquinone is MK-9(H-6), the major cellular lipid is phosphatidylinositol and fatty acids consist mainly of hexadecanoic acid. No mycolic acid was detected. Furthermore, A. graeca 32-07 has been confirmed as a novel producer of the non-ribosomal peptide antibiotic zelkovamycin and we report herein a provisional description of the unique biosynthetic gene cluster.

Item Type: Article
Funders: Biotechnology and Biological Sciences Research Council Doctoral Training Partnership, UK (BBSRC DTP) studentship, UK Research & Innovation (UKRI) Engineering & Physical Sciences Research Council (EPSRC) [EP/L022494/1], University of Nottingham, Universiti Malaya [FP022-2018A]
Uncontrolled Keywords: Lipid-composition; Genome; Acid; Streptomyces; Identification; Classification; Sequences; Names; List; DNA
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms Zaharah Ramly
Date Deposited: 09 Jun 2022 03:47
Last Modified: 09 Jun 2022 03:47

Actions (login required)

View Item View Item