Calcium-Silicate-incorporated Gellan-Chitosan induced osteogenic differentiation in mesenchymal stromal cells

Genasan, Krishnamurithy and Mehrali, Mohammad and Veerappan, Tarini and Talebian, Sepehr and Malliga Raman, Murali and Singh, Simmrat and Swamiappan, Sasikumar and Mehrali, Mehdi and Kamarul, Tunku and Balaji Raghavendran, Hanumantha Rao (2021) Calcium-Silicate-incorporated Gellan-Chitosan induced osteogenic differentiation in mesenchymal stromal cells. Polymers, 13 (19). ISSN 2073-4360, DOI

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Gellan-chitosan (GC) incorporated with CS: 0% (GC-0 CS), 10% (GC-10 CS), 20% (GC-20 CS) or 40% (GC-40 CS) w/w was prepared using freeze-drying method to investigate its physicochemical, biocompatible, and osteoinductive properties in human bone-marrow mesenchymal stromal cells (hBMSCs). The composition of different groups was reflected in physicochemical analyses performed using BET, FTIR, and XRD. The SEM micrographs revealed excellent hBMSCs attachment in GC-40 CS. The Alamar Blue assay indicated an increased proliferation and viability of seeded hBMSCs in all groups on day 21 as compared with day 0. The hBMSCs seeded in GC-40 CS indicated osteogenic differentiation based on an amplified alkaline-phosphatase release on day 7 and 14 as compared with day 0. These cells supported bone mineralization on GC-40 CS based on Alizarin-Red assay on day 21 as compared with day 7 and increased their osteogenic gene expression (RUNX2, ALP, BGLAP, BMP, and Osteonectin) on day 21. The GC-40 CS-seeded hBMSCs initiated their osteogenic differentiation on day 7 as compared with counterparts based on an increased expression of type-1 collagen and BMP2 in immunocytochemistry analysis. In conclusion, the incorporation of 40% (w/w) calcium silicate in gellan-chitosan showed osteoinduction potential in hBMSCs, making it a potential biomaterial to treat critical bone defects.

Item Type: Article
Funders: Fundamental Research Grant Scheme[FRGS/1/2018/SKK08/UM/01/3]
Uncontrolled Keywords: Gellan;Chitosan;Bone;Calcium silicate;Osteoinduction;Bone tissue engineering
Subjects: Q Science > QR Microbiology
R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms Zaharah Ramly
Date Deposited: 15 Jun 2022 07:21
Last Modified: 15 Jun 2022 07:21

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