Detection of enteroviruses during a 2018 hand, foot and mouth disease outbreak in Malaysia

Lee, M. H. P. and Chong, Y. M. and Tay, C. G. and Koh, M. T. and Chem, Y. K. and Noordin, N. and Jahis, R. and Sam, I. C. and Chan, Y. F. (2021) Detection of enteroviruses during a 2018 hand, foot and mouth disease outbreak in Malaysia. Tropical Biomedicine, 38 (1). pp. 150-153. ISSN 0127-5720, DOI

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Hand, foot, and mouth disease ( HFMD) is a common childhood disease caused by enteroviruses. In 2018, a HFMD outbreak in Malaysia affected over 76,000 children. In this study, we used RT-qPCR and CODEHOP PCR to detect the causative agents in 89 clinically diagnosed HFMD patients in Kuala Lumpur and Selangor. Most (62.9%) of the children were below 3 years old. PCR with either assay detected enteroviruses in 84.2% (75/89) and CODEHOP PCR successfully typed 66.7% (50/75) of the enteroviruses. Sequencing of CODEHOP amplicons showed co-circulation of multiple enteroviruses with coxsackievirus A6 (CV-A6) and A16 as the predominant serotypes, but not the neurovirulent enterovirus A71. CV-A6 infection was more common in children less than 12 months old (p=0.01) and was more likely to cause vesicles in the gluteal area (p=0.01) compared to other enteroviruses. Establishing a robust identification method during HFMD outbreaks is important for patient management and public health responses.

Item Type: Article
Funders: Ministry of Higher Education, Malaysia (Fundamental Research Grant Scheme) [Grant No: FRGS/1/2018/SKK11/UM/02/2], inistry of Energy, Science, Technology, Environment and Climate Change (MESTECC), Malaysia [Grant No: 02-01-03-SF1210]
Uncontrolled Keywords: Hand; Foot and mouth disease; HFMD; Enterovirus; Coxsackievirus A6; Malaysia
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine
Depositing User: Ms Zaharah Ramly
Date Deposited: 14 Jun 2022 07:53
Last Modified: 14 Jun 2022 07:53

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