Toxic bloom of Pseudo-nitzschia cuspidata (Bacillariophyceae) and domoic acid contamination of bivalve molluscs in Malaysia Borneo

Teng, Sing Tung and Abdullah, Nursyahida and Hanifah, Afiqah Halmiton and Tan, Suh Nih and Gao, Chunlei and Law, Ing Kuo and Leaw, Chui Pin and Lim, Po Teen (2021) Toxic bloom of Pseudo-nitzschia cuspidata (Bacillariophyceae) and domoic acid contamination of bivalve molluscs in Malaysia Borneo. Toxicon, 202. pp. 132-141. ISSN 0041-0101, DOI

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In March 2018, an algal bloom of Pseudo-nitzschia was detected, for the first time, in a semi-enclosed lagoon in Miri, Sarawak, Malaysia Borneo. The plankton samples were collected for cell enumeration and species identification by electron microscopy and molecular characterization. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was performed to detect and quantify the neurotoxin domoic acid (DA) in both the plankton and shellfish samples. The abundance of Pseudo-nitzschia cells ranged from 5.6 x 10(5) to 3.5 x 10(6) cell L-1 during the bloom event. Morphological observation of the cells by transmission electron microscopy showed that the plankton samples comprised a single Pseudo-nitzschia morphotype resembling P. cuspidata. The ITS2 sequence-structure phylogenetic inference further supported the species identity as Pseudo-nitzschia cuspidata. Low levels of DA were detected in the plankton samples, with cellular DA, particulate DA, and dissolved DA of 257-504 fg DA cell(-1), 676 ng L-1, and 15 ng L-1, respectively. The amount of DA, 8 mu g g(-1) tissue, was found present in the shellfish sample (Magallana sp.) which is below the regulatory limit of 20 mu g DA g(-1) tissue. The study documented, for the first time, DA contamination in shellfish that associated with bloom of P. cuspidata in the Western Pacific region.

Item Type: Article
Funders: Ministry of Education, Singapore[RACER/1/2019/STG03/UNIMAS//1], Universiti Malaysia Sarawak[F07/SGS/1522/2016], Universiti Malaysia Sarawak[I01/SHC/1627/2017]
Uncontrolled Keywords: Amnesic shellfish poisoning;Diatom taxonomy and phylogeny;Harmful algal bloom;New DA record;Pseudo-nitzschia; Shellfish contamination
Subjects: G Geography. Anthropology. Recreation > GC Oceanography
G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > Q Science (General)
Divisions: Deputy Vice Chancellor (Research & Innovation) Office > Institute of Ocean and Earth Sciences
Depositing User: Ms Zaharah Ramly
Date Deposited: 22 Jun 2022 07:12
Last Modified: 22 Jun 2022 07:12

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