Transcriptomic responses to coaggregation between streptococcus gordonii and streptococcus oralis

Choo, Siew Woh and Mohammed, Waleed K. and Mutha, Naresh V. R. and Rostami, Nadia and Ahmed, Halah and Krasnogor, Natalio and Tan, Geok Yuan Annie and Jakubovics, Nicholas S. (2021) Transcriptomic responses to coaggregation between streptococcus gordonii and streptococcus oralis. Applied and Environmental Microbiology, 87 (22). ISSN 0099-2240, DOI

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Cell-cell adhesion between oral bacteria plays a key role in the development of polymicrobial communities such as dental plaque. Oral streptococci such as Streptococcus gordonii and Streptococcus oralis are important early colonizers of dental plaque and bind to a wide range of different oral microorganisms, forming multi-species clumps or ``coaggregates.'' S. gordonii actively responds to coaggregation by regulating gene expression. To further understand these responses, we assessed gene regulation in S. gordonii and S. oralis following coaggregation in 25% human saliva. Coaggregates were formed by mixing, and after 30 min, RNA was extracted for dual transcriptome sequencing (RNA-Seq) analysis. In S. oralis, 18 genes (6 upregulated and 12 downregulated) were regulated by coaggregation. Significantly downregulated genes encoded functions such as amino acid and antibiotic biosynthesis, ribosome, and central carbon metabolism. In total, 28 genes were differentially regulated in Streptococcus gordonii (25 upregulated and 3 downregulated). Many genes associated with transporters and a two-component (NisK/SpaK) regulatory system were upregulated following coaggregation. Our comparative analyses of S. gordonii-S. oralis with different previously published S. gordonii pairings (S. gordonii-Fusobacterium nucleatum and S. gordonii-Veillonella parvula) suggest that the gene regulation is specific to each pairing, and responses do not appear to be conserved. This ability to distinguish between neighboring bacteria may be important for S. gordonii to adapt appropriately during the development of complex biofilms such as dental plaque. IMPORTANCE Dental plaque is responsible for two of the most prevalent diseases in humans, dental caries and periodontitis. Controlling the formation of dental plaque and preventing the transition from oral health to disease requires a detailed understanding of microbial colonization and biofilm development. Streptococci are among the most common colonizers of dental plaque. This study identifies key genes that are regulated when oral streptococci bind to one another, as they do in the early stages of dental plaque formation. We show that specific genes are regulated in two different oral streptococci following the formation of mixed-species aggregates. The specific responses of S. gordonii to coaggregation with S. oralis are different from those to coaggregation with other oral bacteria. Targeting the key genes that are upregulated during interspecies interactions may be a powerful approach to control the development of biofilm and maintain oral health.

Item Type: Article
Funders: Ministry of Higher Education and Scientific Research (Iraq), UK Research & Innovation (UKRI) Engineering & Physical Sciences Research Council (EPSRC) [EP/J004111/2] [EP/N031962/1], NSFC International Young Scientists Fund [31750110452], WenzhouKean University [5000105]
Uncontrolled Keywords: Bioinformatics; Coaggregation; Oral streptococci; Streptococcus gordonii; Streptococcus oralis; Transcriptome; biofilms
Subjects: Q Science > QR Microbiology
T Technology > TP Chemical technology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms Zaharah Ramly
Date Deposited: 07 Jul 2022 01:42
Last Modified: 07 Jul 2022 01:42

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