Real-time NMR monitoring of spatially segregated enzymatic reactions in multilayered hydrogel assemblies

Nordin, Nurdiana and Bordonali, Lorenzo and Davoodi, Hossein and Ratnawati, Novindi Dwi and Gygli, Gudrun and Korvink, Jan G. and Badilita, Vlad and MacKinnon, Neil (2021) Real-time NMR monitoring of spatially segregated enzymatic reactions in multilayered hydrogel assemblies. Angewandte Chemie-International Edition, 60 (35). pp. 19176-19182. ISSN 1433-7851, DOI

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Compartmentalized chemical reactions at the microscale are important in biotechnology, yet monitoring the molecular content at these small scales is challenging. To address this challenge, we integrate a compact, reconfigurable reaction cell featuring electrochemical functionality with high-resolution NMR spectroscopy. We demonstrate the operation of this system by monitoring the activity of enzymes immobilized in chemically distinct layers within a multi-layered chitosan hydrogel assembly. As a benchmark, we observed the parallel activities of urease (Urs), catalase (Cat), and glucose oxidase (GOx) by monitoring reagent and product concentrations in real-time. Simultaneous monitoring of an independent enzymatic process (Urs) together with a cooperative process (GOx + Cat) was achieved, with chemical conversion modulation of the GOx + Cat process demonstrated by varying the order in which the hydrogel was assembled.

Item Type: Article
Funders: German Research Foundation (DFG) [Grant No: DFG MA 6653/1-1 & DFG BA 4275/4-1], Universiti Malaya, Federal Ministry of Education & Research (BMBF), Baden-Wgrttemberg Ministry of Science as part of the Excellence Strategy of the German Federal and State Governments, Helmholtz program "Materials Systems Engineering" under the topic "Adaptive and Bioinstructive Materials Systems", EU2020 FET grant (TiSuMR) [Grant No: 737043], ERC-SyG (HiSCORE) [Grant No: 951459], German Research Foundation (DFG) European Commission [Grant No: KO 1883/39-1], German Excellence Intitiative, Helmholtz Association, Karlsruhe Institute of Technology (KIT), Projekt DEAL
Uncontrolled Keywords: bioMEMS; Catalase; Chitosan; Electrodeposition; Glucose oxidase
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Science > Department of Chemistry
Depositing User: Ms Zaharah Ramly
Date Deposited: 27 Jun 2022 01:38
Last Modified: 27 Jun 2022 01:38

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