The potential use of chlorhexidine (CHX) and hexetidine-containing mouth rinse in maintaining toothbrush sterility

Himratul-Aznita, Wan Harun and Razak, Fathilah Abdul (2006) The potential use of chlorhexidine (CHX) and hexetidine-containing mouth rinse in maintaining toothbrush sterility. Journal of Medical Sciences, 6 (1). pp. 59-62. ISSN 1682-4474, DOI

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The study was carried out with the aim of demonstrating quantitatively the presence of microorganisms adhered to toothbrush bristles and to determine the potential of using antimicrobial agent (such as chlorhexidine gluconate (CHX) and hexetidine (HX)) in commercialized mouth rinses to reduce microbial contamination. The study was carried out by enumerating the total colony counts of bristles-adhered microbes after three weeks of normal oral hygiene followed by rinsing the toothbrushes with CHX, HX, tap water and deionized water independently following a strict planned schedule. Rinsing toothbrush with tap water was included in the study as a control due to the normal way of cleaning toothbrush after use in every home. Whereas, sterilized deionized water do not contain any ions, minerals and is microbes-free. The total colony counts of microbes obtained from the toothbrush rinsed with tap water, deionized water, CHX and HX were 62.6×106 CFU mL-1, 74.4×106 CFU mL-1, 2.4×106 CFU mL-1 and 7.6×106 CFU mL-1, respectively. Staphylococcus aureus, Actinomyces naeslundii and Clostridium sp. were isolated from toothbrush rinsed with tap water. Staphylococcus aureus and Peptostreptococcus sp. were obtained from toothbrush rinsed with deionized water. Actinomyces sp. and Clostridium sp. were recovered from toothbrush rinsed with CHX and only Staphylococcus aureus was obtained from toothbrush rinsed with HX. Although toothbrush rinsed with mouth rinses containing antimicrobial agent such as CHX and HX still harbour microorganisms, but the microbial load has been very much lowered compared to the control toothbrush. Thus, this indicates that toothbrush rinsing with mouth rinse after the normal oral hygiene is very convenient and cost effective to reduce toothbrush contamination.

Item Type: Article
Additional Information: Cited By (since 1996): 1 Export Date: 25 June 2012 Source: Scopus Language of Original Document: English Correspondence Address: Himratul-Aznita, W.H.; Department of Oral Biology, Faculty of Dentistry, 50603 Kuala Lumpur, Malaysia Chemicals/CAS: chlorhexidine, 3697-42-5, 55-56-1; hexetidine, 141-94-6 References: Dayoub, D., Rusiko, D.B., Gross, A., Microbial contamination of toothbrushes (1977) J. Dent. Res., 56, p. 706; Taji, S.S., Rogers, A.H., The microbial contamination of toothbrushes. A pilot study (1998) Aust. Dent. J., 43, pp. 128-130; Glass, R.T., Lare, M.M., Toothbrush contamination: A potential health risk (1986) Quintessence Intl., 17, pp. 39-42; Svanberg, M., Contamination of toothpaste and toothbrush by Streptococcus mutans (1978) Scand. J. Dent. Res., 86, pp. 412-414; Caudry, S.D., Klitorinos, A., Chan, E.C., Contaminated toothbrushes and their disinfection (1995) J. Can. Dent. Assoc., 61, pp. 511-516; Meier, S., Collier, C., Scaletta, M.G., Stephens, J., Kimbrough, R., Kettering, J.D., An in vitro investigation of the efficiency of CPC for use in toothbrush decontamination (1996) J. Dent. Hyg., 70, pp. 161-165; Warren, D.P., Goldschmidt, M.C., Thompson, M.B., Adler-Storthz, K., Keene, H.J., The effects of toothpastes on the residual microbial contamination of toothbrushes (2001) J. Am. Dent. Assoc., 132, pp. 1241-1245; Hefti, A.F., Huber, B., The effect on early plaque formation, gingivitis and salivary bacterial counts of mouthwashes containing hexetidine/zinc, aminefluoride/tin or chlorhexidine (1987) J. Clin. Periodontol., 14, pp. 515-518
Uncontrolled Keywords: Chlorhexidine Hexetidine Mouth rinse Tooth brushing tap water Actinomyces naeslundii antimicrobial activity article bacterial count bacterium colony bacterium contamination bacterium isolate bacterium isolation Clostridium controlled study cost effectiveness analysis human instrument sterilization microbial contamination mouth hygiene Peptostreptococcus quantitative analysis risk reduction Staphylococcus aureus toothbrush
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry > Dept of Oral Biology
Depositing User: Mr Ahmad Azwan Azman
Date Deposited: 29 Jun 2012 02:04
Last Modified: 23 Dec 2019 07:28

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