Epigenetic distribution of recombinant plant chromosome fragments in a human-arabidopsis hybrid cell line

Liaw, YengMun and Liu, Yikun and Teo, CheeHow and Capal, Petr and Wada, Naoki and Fukui, Kiichi and Dolezel, Jaroslav and Ohmido, Nobuko (2021) Epigenetic distribution of recombinant plant chromosome fragments in a human-arabidopsis hybrid cell line. International Journal of Molecular Sciences, 22 (11). ISSN 1422-0067, DOI https://doi.org/10.3390/ijms22115426.

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Methylation systems have been conserved during the divergence of plants and animals, although they are regulated by different pathways and enzymes. However, studies on the interactions of the epigenomes among evolutionarily distant organisms are lacking. To address this, we studied the epigenetic modification and gene expression of plant chromosome fragments (similar to 30 Mb) in a human Arabidopsis hybrid cell line. The whole-genome bisulfite sequencing results demonstrated that recombinant Arabidopsis DNA could retain its plant CG methylation levels even without functional plant methyltransferases, indicating that plant DNA methylation states can be maintained even in a different genomic background. The differential methylation analysis showed that the Arabidopsis DNA was undermethylated in the centromeric region and repetitive elements. Several Arabidopsis genes were still expressed, whereas the expression patterns were not related to the gene function. We concluded that the plant DNA did not maintain the original plant epigenomic landscapes and was under the control of the human genome. This study showed how two diverging genomes can coexist and provided insights into epigenetic modifications and their impact on the regulation of gene expressions between plant and animal genomes.

Item Type: Article
Funders: Kobe University Visiting Researcher Project" from Kobe University, ERDF project "Plants as a tool for sustainable global development [Grant No: CZ.02.1.01/0.0/0.0/16_019/0000827], Mobility Plus Project [Grant No: JSPS-18-07 & JPJSBP120203507], Innovative Asia by JICA
Uncontrolled Keywords: Arabidopsis genome; DNA methylation; Epigenome; human-plant hybrid cell line; Gene expression; Whole-genome bisulfite sequencing (WGBS)
Subjects: Q Science > QD Chemistry
Divisions: Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture
Depositing User: Ms Zaharah Ramly
Date Deposited: 12 Jul 2022 04:34
Last Modified: 12 Jul 2022 04:34
URI: http://eprints.um.edu.my/id/eprint/33922

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