Oecophyllibacter saccharovorans gen. nov. sp. nov., a bacterial symbiont of the weaver ant Oecophylla smaragdina

Chua, Kah-Ooi and See-Too, Wah-Seng and Tan, Jia-Yi and Song, Sze-Looi and Yong, Hoi-Sen and Yin, Wai-Fong and Chan, Kok-Gan (2020) Oecophyllibacter saccharovorans gen. nov. sp. nov., a bacterial symbiont of the weaver ant Oecophylla smaragdina. Journal of Microbiology, 58 (12). pp. 988-997. ISSN 1225-8873, DOI https://doi.org/10.1007/s12275-020-0325-8.

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In this study, bacterial strains Ha5(T), Ta1, and Jb2 were isolated from different colonies of weaver ant Oecophylla smaragdina. They were identified as bacterial symbionts of the ant belonging to family Acetobacteraceae and were distinguished as different strains based on distinctive random-amplified polymorphic DNA (RAPD) fingerprints. Cells of these bacterial strains were Gram-negative, rod-shaped, aerobic, non-motile, catalase-positive and oxidase-negative. They were able to grow at 15-37 degrees C (optimum, 28-30 degrees C) and in the presence of 0-1.5% (w/v) NaCl (optimum 0%). Their predominant cellular fatty acids were C-18:1 omega 7c, C-16:0, C-19:0 omega 8c cyclo, C-14:0, and C-16:0 2-OH. Strains Ha5(T), Ta1, and Jb2 shared highest 16S rRNA gene sequence similarity (94.56-94.63%) with Neokomagataea tanensis NBRC106556(T) of family Acetobacteraceae. Both 16S rRNA gene sequence-based phylogenetic analysis and core gene-based phylogenomic analysis placed them in a distinct lineage in family Acetobacteraceae. These bacterial strains shared higher than species level thresholds in multiple overall genome-relatedness indices which indicated that they belonged to the same species. In addition, they did not belong to any of the current taxa of Acetobacteraceae as they had low pairwise average nucleotide identity (< 71%), in silico DNA-DNA hybridization (< 38%) and average amino acid identity (< 67%) values with all the type members of the family. Based on these results, bacterial strains Ha5(T), Ta1, and Jb2 represent a novel species of a novel genus in family Acetobacteaceae, for which we propose the name Oecophyllibacter saccharovorans gen. nov. sp. nov., and strain Ha5(T) as the type strain.

Item Type: Article
Funders: MyBrain15 Postgraduate Scholarship Programme [Grant No: KPT(B)900909146137], Bright Sparks Program of the University of Malaya, University of Malaya Research Grants (FRGS) [Grant No: FP022-2018A], University of Malaya High Impact Research Grants (UM-MOHE HIR Grant) [Grant No: UM.C/625/1/HIR/MOHE/CHAN/14/1 & H-50001-A000027], UM-MOHE HIR Grant [Grant No: UM.C/625/1/HIR/MOHE/CHAN/01 & A-000001-50001], Postgraduate Research (PPP) Grant [Grant No: PG089-2015B]
Uncontrolled Keywords: Oecophyllibacter saccharovorans; Acetobacteraceae; Insect; Phylogenomics; Percentage of conserved protein
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Deputy Vice Chancellor (Research & Innovation) Office > Institute of Ocean and Earth Sciences
Depositing User: Mrs. Siti Mawarni Salim
Date Deposited: 20 Oct 2022 03:02
Last Modified: 20 Oct 2022 03:02
URI: http://eprints.um.edu.my/id/eprint/31605

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