Serotype analysis of rotaviruses from different locations in Malaysia.

Rasool, N.B. and Green, K.Y. and Kapikian, A.Z. (1993) Serotype analysis of rotaviruses from different locations in Malaysia. Journal of Clinical Microbiology, 31 (7). pp. 1815-9. ISSN 0095-1137, DOI 8394376.

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The distribution of rotavirus G (VP7) serotypes circulating in four locations in Malaysia, representing three geographical areas, was evaluated in 341 RNA-positive stool specimens obtained discontinuously between 1977 and 1988 from infants and young children under the age of five years who were hospitalized with acute gastroenteritis. A total of 306 specimens (256 stool suspensions and 50 that were adapted to growth in tissue culture) that were rotavirus positive by the confirmatory enzyme-linked immunosorbent assay (ELISA) were examined for serotype by ELISA utilizing monoclonal antibodies to rotavirus G serotype 1, 2, 3, 4, or 9. One hundred eighty (59%) of the 306 specimens could be serotyped; of these 180 specimens, 71% were serotype 4, 15% were serotype 1, 4% were serotype 2, and 4% were serotype 3. Serotype 9 rotavirus was not detected. Most (71%) of the specimens tested were obtained in 1988, when serotype 4 predominated in three locations in West Malaysia; no single serotype was predominant in a limited number of specimens from East Malaysia.

Item Type: Article
Uncontrolled Keywords: Antibodies, Monoclonal; Antigens, Viral/isolation & purification; Child, Preschool; Enzyme-Linked Immunosorbent Assay;
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine
Depositing User: Mr. Faizal Hamzah
Date Deposited: 02 Mar 2011 07:02
Last Modified: 04 Aug 2017 07:31

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