Sandwich dot-immunogold filtration assay (DIGFA) for specific immunodiagnosis of active neuroangiostrongyliasis

Eamsobhana, Praphathip and Tungtrongchitr, Anchalee and Yong, Hoi-Sen and Prasartvit, Anchana and Wanachiwanawin, Darawan and Gan, Xiao-Xian (2021) Sandwich dot-immunogold filtration assay (DIGFA) for specific immunodiagnosis of active neuroangiostrongyliasis. Parasitology, 148 (2). pp. 234-239. ISSN 0031-1820, DOI

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Serological tests may yield false-negative results for specific antibodies detection before or at the early seroconversion phase. Tests that detect circulating antigens of Angiostrongylus cantonensis would therefore be of value in diagnosis to distinguish current or past infection. Here, a quick, easy to perform, portable and inexpensive diagnostic device for detection of 31-kDa A. cantonensis specific antigens had been developed. This sandwich dot-immunogold filtration assay (AcDIGFA(Ag)), for detecting active angiostrongyliasis was produced using anti-A. cantonensis polyclonal antibody dotted on the nitrocellulose membrane as a capture agent and colloidal gold-labelled anti-31 kDa A. cantonensis antibody as a detection agent. A well-defined pink dot, indicating positivity, was seen readily by naked eye within 10-15 min. The AcDIGFA(Ag) detected A. cantonensis-specific antigens in cerebrospinal fluid samples from 4 out of 10 serologically confirmed angiostrongyliasis cases and 2 out of 5 suspected cases with negative anti-A. cantonensis antibodies. Among the 19 patient sera with A. cantonensis infection, 2 showed positive reaction by AcDIGFA(Ag). No positive AcDIGFA(Ag) reaction was observed in all the serum samples with other parasitic diseases, and the healthy controls. The present `AcDIGFA(Ag)' enables rapid qualitative detection of the specific 31-kDa antigens of A. cantonensis in clinical samples with potential for application even under resource-limited settings.

Item Type: Article
Funders: Department of Disease Control, Ministry of Public Health, Thailand, Universiti Malaya [H-5620009]
Additional Information: 6th International Workshop on Angiostrongylus and Angiostrongliasis, Hilo, HI, JAN, 2020
Uncontrolled Keywords: Angiostrongylus cantonensis; Sandwich dot-immunogold filtration assay; DIGFA; Antigen detection; 31-kDa antigen; active angiostrongyliasis
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms Zaharah Ramly
Date Deposited: 15 Aug 2022 07:57
Last Modified: 15 Aug 2022 07:57

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