Plasmid localization of sole rrn operon in genomes of Oecophyllibacter saccharovorans (Acetobacteraceae)

Chua, Kah-Ooi and See-Too, Wah-Seng and Yong, Hoi-Sen and Song, Sze-Looi and Yin, Wai-Fong and Chan, Kok-Gan (2021) Plasmid localization of sole rrn operon in genomes of Oecophyllibacter saccharovorans (Acetobacteraceae). Plasmid, 114. ISSN 0147-619X, DOI

Full text not available from this repository.


The bacterium Oecophyllibacter saccharovorans of family Acetobacteraceae is a symbiont of weaver ant Oecophylla smaragdina. In our previous study, we published the finding of novel O. saccharovorans strains Ha5(T), Ta1 and Jb2 (Chua et al. 2020) but their plasmid sequences have not been reported before. Here, we demonstrate for the first time that the sole rrn operon of their genomes was detected on a 6.6 kb circular replicon. This replicon occurred in high copy number, much smaller size and lower G + C content than the main chromosome. Based on these features, the 6.6 kb circular replicon was regarded as rrn operon-containing plasmid. Further restriction analysis on the plasmids confirmed their circular conformation. A Southern hybridization analysis also corroborated the presence of 16S rRNA gene and thus the rrn operon on a single locus in the genome of the O. saccharovorans strains. However, similar genome architecture was not observed in other closely related bacterial strains. Additional survey also detected no plasmid-borne rrn operon in available genomes of validly described taxa of family Acetobacteraceae. To date, plasmid localization of rrn operon is rarely documented. This study reports the occurrence of rrn operon on the smallest bacterial plasmid in three O. saccharovorans strains and discusses its possible importance in enhancing their competitive fitness as bacterial symbiont of O. smaragdina.

Item Type: Article
Funders: University of Malaya Research Grants (FRGS grant) [FP022-2018A], University of Malaya High Impact Research Grants (UM-MOHE HIR Grant) [UM.C/625/1/HIR/MOHE/CHAN/14/1] [H-50001-A000027] [UM.C/625/1/HIR/MOHE/CHAN/01] [A-000001-50001]
Uncontrolled Keywords: Oecophyllibacter saccharovorans; Rrn operon; Plasmid; Genome organization
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions: Institute of Advanced Studies
Depositing User: Ms Zaharah Ramly
Date Deposited: 08 Aug 2022 06:25
Last Modified: 08 Aug 2022 06:25

Actions (login required)

View Item View Item