Experimental study on plasmodium knowlesi normocyte binding protein xa region ii (pknbpxaii) for erythrocyte binding

Wong, K. C. and Lai, M. Y. and De Silva, J. R. and Cheong, Fei Wen and Fong, Mun Yik and Lau, Yee Ling (2021) Experimental study on plasmodium knowlesi normocyte binding protein xa region ii (pknbpxaii) for erythrocyte binding. Tropical Biomedicine, 38 (2). pp. 143-148. ISSN 0127-5720, DOI https://doi.org/10.47665/tb.38.2.051.

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Normocyte binding protein Xa (NBPXa) has been implied to play a significant role in parasite invasion of human erythrocytes. Previous phylogenetic studies have reported the existence of three types of NBPXa for Plasmodium knowlesi (PkNBPXa). PkNBPXa region II (PkNBPXaII) of type 1, type 2 and type 3 were expressed on mammalian cell surface and interacted with human and macaque (Macaca fascicularis) erythrocytes. The binding activities of PkNBPXaII towards human and macaque erythrocytes were evaluated using erythrocyte-binding assay (EBA). Three parameters were evaluated to achieve the optimal protein expression of PkNBPXaII and erythrocyte binding activity in EBA: types of mammalian cells, post transfection time and erythrocyte incubation time. COS-7, HEK-293, and CHO-K1 cells showed successful expression of PkNBPXaII, despite the protein expression is weak compared to the positive control. COS-7 was used in EBA. All three types of PkNBPXaII showed rosette formation with macaque erythrocytes but not with human erythrocytes. Future studies to enhance the PkNBPXaII expression on surface of mammalian cells is indeed needed in order to elucidate the specific role of PkNBPXaII in erythrocytes invasion.

Item Type: Article
Funders: Ministry of Education, Malaysia (LR002A-2018)
Uncontrolled Keywords: Plasmodium knowlesi; Normocyte binding protein Xa; Cloning; Protein expression; Erythrocyte binding
Subjects: Q Science > QL Zoology
R Medicine > RC Internal medicine
Divisions: Faculty of Medicine
Depositing User: Ms Zaharah Ramly
Date Deposited: 12 Apr 2022 07:19
Last Modified: 12 Apr 2022 07:19
URI: http://eprints.um.edu.my/id/eprint/28318

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