Freeze-drying improves DNA yield from teeth

Rahmat, Rabiah Al-Adawiyah. and Humphries, Melissa A. and Linacre, Adrian M. T. and Malik, Arif and Saedon, Nor A. and Austin, Jeremy J. (2021) Freeze-drying improves DNA yield from teeth. Forensic Science International, 326. ISSN 0379-0738, DOI

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The common method of preparing teeth prior to DNA extraction involves cleaning, decontamination, drying and pulverisation. Moisture in post-mortem teeth can promote bacterial growth and hydrolytic damage that could contribute to DNA degradation, whilst also possibly reducing the efficiency of sample pulverisation and DNA release. Here we compared DNA extraction from pig teeth, with-and without freeze-drying, to examine the impact of removing moisture on DNA yield. Quantitative real-time polymerase chain reaction (qPCR) was used to quantify an 83 bp mitochondrial DNA fragment and two nuclear DNA fragments of 82 bp and 150 bp. The comparative results showed that sample preparation with freeze-drying resulted in a higher DNA yield without compromising the DNA quality. This study highlights the advantage of in-corporating a freeze-drying to improve the DNA yield and minimising the loss of DNA during sample preparation of teeth. (c) 2021 Elsevier B.V. All rights reserved.

Item Type: Article
Funders: School of Biological Sciences, the University of Adelaide
Uncontrolled Keywords: Teeth; Sample preparation; Freeze-drying; DNA extraction; Quantitative polymerase chain reaction
Subjects: R Medicine > RA Public aspects of medicine > RA1001 Forensic Medicine. Medical jurisprudence. Legal medicine
Divisions: Faculty of Dentistry > Dept of Oral & Maxillofacial Surgery
Depositing User: Ms Zaharah Ramly
Date Deposited: 12 Apr 2022 07:24
Last Modified: 12 Apr 2022 07:24

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