In vitro and in vivo evaluation of a nanofiber wound dressing loaded with melatonin

Mirmajidi, Tahereh and Chogan, Faraz and Rezayan, Ali Hossein and Sharifi, Ali Mohammad (2021) In vitro and in vivo evaluation of a nanofiber wound dressing loaded with melatonin. International Journal of Pharmaceutics, 596. ISSN 0378-5173, DOI

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Wound healing is a complicated process that takes a long time to complete. The three-layer nanofiber wound dressing containing melatonin is highly expected to show remarkable wound repair by reducing the wound healing time. In this study, chitosan (Cs)-polycaprolactone (PCL)/ polyvinylalcohol (PVA)-melatonin (MEL)/ chitosan-polycapmlactone three-layer nanofiber wound dressing was prepared by electrospinning for melatonin sustained release. The characteristics of the wound dressing were further evaluated. The wound dressing had a high water uptake after 24 h (401%), and the water contact angle results showed that it had hydrophilicity effect that supported the cell attachment. The wound healing effect of wound dressing was examined using a full-thickness excisional model of rat skin by the local administration of MEL. The gene expressions of transforming growth factor-beta (TGF-beta 1), alpha-smooth muscle actin (alpha-SMA), collagen type I (COL1A1), and collagen type III (COL3A1) were further studied. The histopathological evaluation showed the complete regeneration of the epithelial layer, remodeling of wounds, collagen synthesis, and reduction in inflammatory cells. The NF + 20% MEL significantly increased TGF-beta 1, COL1A1, COL3A1, and alpha-SMA mRNA expressions. This wound dressing may have a considerable potential as a wound dressing to accelerate the wound healing.

Item Type: Article
Funders: University of Tehran (18/06/28445)
Uncontrolled Keywords: Melatonin; Drug delivery; Nanofiber wound dressing; Polycaprolactone; Polyvinyl alcohol; Chitosan; Electrospinning
Subjects: R Medicine > RM Therapeutics. Pharmacology
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 15 Mar 2022 08:24
Last Modified: 15 Mar 2022 08:24

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