Harnessing CRISPR-Cas to combat COVID-19: From diagnostics to therapeutics

Chan, Kok Gan and Ang, Geik Yong and Yu, Choo Yee and Yean, Chan Yean (2021) Harnessing CRISPR-Cas to combat COVID-19: From diagnostics to therapeutics. Life-Basel, 11 (11). ISSN 2075-1729, DOI https://doi.org/10.3390/life11111210.

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The coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), remains a global threat with an ever-increasing death toll even after a year on. Hence, the rapid identification of infected individuals with diagnostic tests continues to be crucial in the on-going effort to combat the spread of COVID-19. Viral nucleic acid detection via real-time reverse transcription polymerase chain reaction (rRT-PCR) or sequencing is regarded as the gold standard for COVID-19 diagnosis, but these technically intricate molecular tests are limited to centralized laboratories due to the highly specialized instrument and skilled personnel requirements. Based on the current development in the field of diagnostics, the programmable clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) system appears to be a promising technology that can be further explored to create rapid, cost-effective, sensitive, and specific diagnostic tools for both laboratory and point-of-care (POC) testing. Other than diagnostics, the potential application of the CRISPR-Cas system as an antiviral agent has also been gaining attention. In this review, we highlight the recent advances in CRISPR-Cas-based nucleic acid detection strategies and the application of CRISPR-Cas as a potential antiviral agent in the context of COVID-19.

Item Type: Article
Uncontrolled Keywords: Coronavirus; COVID-19; Isothermal amplification; Antiviral; CRISPR-Dx
Subjects: Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 04 Mar 2022 02:20
Last Modified: 04 Mar 2022 02:20
URI: http://eprints.um.edu.my/id/eprint/26459

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