A pan-specific antiserum produced by a novel immunization strategy shows a high spectrum of neutralization against neurotoxic snake venoms

Ratanabanangkoon, Kavi and Tan, Kae Yi and Pruksaphon, Kritsada and Klinpayom, Chaiya and Gutiérrez, José María and Quraishi, Naeem H. and Tan, Choo Hock (2020) A pan-specific antiserum produced by a novel immunization strategy shows a high spectrum of neutralization against neurotoxic snake venoms. Scientific Reports, 10 (1). p. 11261. ISSN 2045-2322, DOI https://doi.org/10.1038/s41598-020-66657-8.

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Official URL: https://doi.org/10.1038/s41598-020-66657-8


Snakebite envenomation is a neglected tropical disease of high mortality and morbidity largely due to insufficient supply of effective and affordable antivenoms. Snake antivenoms are mostly effective against the venoms used in their production. It is thus crucial that effective and affordable antivenom(s) with wide para-specificity, capable of neutralizing the venoms of a large number of snakes, be produced. Here we studied the pan-specific antiserum prepared previously by a novel immunization strategy involving the exposure of horses to a ‘diverse toxin repertoire’ consisting of 12 neurotoxic Asian snake toxin fractions/ venoms from six species. This antiserum was previously shown to exhibit wide para-specificity by neutralizing 11 homologous and 16 heterologous venoms from Asia and Africa. We now show that the antiserum can neutralize 9 out of 10 additional neurotoxic venoms. Altogether, 36 snake venoms belonging to 10 genera from 4 continents were neutralized by the antiserum. Toxin profiles previously generated using proteomic techniques of these 36 venoms identified α-neurotoxins, β-neurotoxins, and cytotoxins as predominant toxins presumably neutralized by the antiserum. The bases for the wide para-specificity of the antiserum are discussed. These findings indicate that it is feasible to generate antivenoms of wide para-specificity against elapid neurotoxic venoms from different regions in the world and raises the possibility of a universal neurotoxic antivenom. This should reduce the mortality resulting from neurotoxic snakebite envenomation. © 2020, The Author(s).

Item Type: Article
Funders: A research grant (no. IM 2011-01 to KR) from the Chulabhorn Research Institute, A research grant from the Ministry of Higher Education, Government of Malaysia (grant no. FRGS/1/2019/SKK08/ UM/02/2)
Uncontrolled Keywords: Venom Antiserum; Bothrops; Snake Bites
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 13 Aug 2020 02:47
Last Modified: 13 Aug 2020 02:47
URI: http://eprints.um.edu.my/id/eprint/25323

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