Cellular Localization of gdnf in Adult Zebrafish Brain

Wong, Chee Ern David and Hua, Khang and Monis, Simon and Norazit, Anwar and Noor, Suzita Mohd and Ekker, Marc (2020) Cellular Localization of gdnf in Adult Zebrafish Brain. Brain Sciences, 10 (5). p. 286. ISSN 2076-3425, DOI https://doi.org/10.3390/brainsci10050286.

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Official URL: https://doi.org/10.3390/brainsci10050286


Glial cell line-derived neurotrophic factor (GDNF) was initially described as important for dopaminergic neuronal survival and is involved in many other essential functions in the central nervous system. Characterization of GDNF phenotype in mammals is well described; however, studies in non-mammalian vertebrate models are scarce. Here, we characterized the anatomical distribution of gdnf -expressing cells in adult zebrafish brain by means of combined in situ hybridization (ISH) and immunohistochemistry. Our results revealed that gdnf was widely dispersed in the brain. gdnf transcripts were co-localized with radial glial cells along the ventricular area of the telencephalon and in the hypothalamus. Interestingly, Sox2 positive cells expressed gdnf in the neuronal layer but not in the ventricular zone of the telencephalon. A subset of GABAergic precursor cells labeled with dlx6a-1.4kbdlx5a/6a: green fluorescence protein (GFP) in the pallium, parvocellular preoptic nucleus, and the anterior and dorsal zones of the periventricular hypothalamus also showed expression with gdnf mRNA. In addition, gdnf signals were detected in subsets of dopaminergic neurons, including those in the ventral diencephalon, similar to what is seen in mammalian brain. Our work extends our knowledge of gdnf action sites and suggests a potential role for gdnf in adult brain neurogenesis and regeneration. © 2020 by the authors.

Item Type: Article
Funders: Malaysian Ministry of Higher Education Fundamental Research Grant Scheme (FP027-2014A), Natural Sciences and Engineering Research Council of Canada grant (121795), University of Malaya Postgraduate Research grant (PG246-2015B), Special Research Assistance grant (BKS064-2017), Research University Grant (Faculty of Medicine RU) (GPF014C-2018), Malaysian Ministry of Higher Education MyPhD program, Malaysian Society of Neurosciences Education Grant (ID: 02246)
Uncontrolled Keywords: gdnf; radial glial cells; Sox2; neuronal progenitor cells; dlx; GABAergic neurons; dopaminergic neurons
Subjects: Q Science > QH Natural history
R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 10 Jun 2020 00:35
Last Modified: 10 Jun 2020 00:35
URI: http://eprints.um.edu.my/id/eprint/24761

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