A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth

Ahmad, Islamudin and Ambarwati, Neneng Siti Silfi and Elya, Berna and Omar, Hanita and Mulia, Kamarza and Yanuar, Arry and Negishi, Osamu and Mun'im, Abdul (2019) A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth. Asian Pacific Journal of Tropical Biomedicine, 9 (6). pp. 257-262. ISSN 2221-1691, DOI https://doi.org/10.4103/2221-1691.260398.

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Official URL: https://doi.org/10.4103/2221-1691.260398


Objective: To isolate, identify, and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth herbs. Methods: A dried sample of Peperomia pellucida herb was successively macerated with n-hexane and ethyl acetate. The ethyl acetate extract solution was evaporated to obtain the crude extract. Vacuum liquid column chromatography and thin layer chromatography were performed to obtain two pure compounds. Then, both compounds were elucidated and identified using the spectroscopic method. Angiotensin-converting enzyme inhibitory activity studies of both compounds were determined using angiotensin-converting enzyme kit WST-1 with spectrophotometer microplate reader 96-well at 450 nm wavelength. Results: Two bioactive compounds were successfully isolated from Peperomia pellucida herb, including a new compound of 2,3,5-trimethoxy-9-(12,14,15-trimethoxybenzyl)-1H-indene and pellucidin A. Both compounds demonstrated angiotensin-converting enzyme inhibitory activity, with IC50 values of 72 μM (27.95 μg/mL) and 11 μM (4.4 μg/mL), respectively. Conclusions: In the present study, two active angiotensin-converting enzyme inhibitors were successfully isolated and purified from Peperomia pellucida which is used as an antihypertensive in traditional medicine, and support its use as an angiotensin-converting enzyme-inhibiting drug. © 2019 Wolters Kluwer Medknow Publications. All rights reserved.

Item Type: Article
Funders: “Hibah Tugas Akhir Mahasiswa Doktor (TADOK) Tahun 2018” Directorate of Research and Humanity Engagement Universitas Indonesia (grant number: 1234/UN2.R3.1/HKP.05.00/2018)
Uncontrolled Keywords: 2,3,5-trimethoxy-9-(12,14,15-trimethoxybenzyl)-1H-indene; Angiotensin-converting enzyme inhibitor; Pellucidin A; Peperomia pellucida (L) Kunth
Subjects: Q Science > QD Chemistry
R Medicine > RS Pharmacy and materia medica
Divisions: Centre for Foundation Studies in Science > Chemistry Division
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 19 May 2020 03:26
Last Modified: 19 May 2020 03:26
URI: http://eprints.um.edu.my/id/eprint/24349

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