Zika virus modulates blood-brain barrier of brain microvascular endothelial cells

Ismail, A.A. and Mahboob, Tooba and Samudi, Chandramathi and Sekaran, Shamala Devi (2019) Zika virus modulates blood-brain barrier of brain microvascular endothelial cells. Tropical Biomedicine, 36 (4). pp. 888-897. ISSN 0127-5720,

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Official URL: http://msptm.org/files/Vol36No4/888-897-Samudi-Raj...


Zika virus (ZIKV) is a mosquito-borne Flaviviruses. ZIKV is known to cause birth defect in pregnant women, especially microcephaly in the fetus. Hence, more study is required to understand the infection of Zika virus towards human brain microvascular endothelial cells (MECs). In this study, brain MECs were infected with ZIKV at MOI of 1 and 5 in vitro. The changes in barrier function and membrane permeability of ZIKV-infected brain MECs were determined using electric cell-substrate impedance sensing (ECIS) system followed by gene expression of ZIKV-infected brain MECs at 24 hours post infection using one-color gene expression microarray. The ECIS results demonstrated that ZIKV infection enhances vascular leakage by increasing cell membrane permeability via alteration of brain MECs barrier function. This was further supported by high expression of proinflammatory cytokine genes (lnc-IL6-2, TNFAIP1 and TNFAIP6), adhesion molecules (CERCAM and ESAM) and growth factor (FIGF). Overall, findings of this study revealed that ZIKV infection could alter the barrier function of brain MECs by altering adhesion molecules and inflammatory response. © 2019, Malaysian Society for Parasitology. All rights reserved.

Item Type: Article
Funders: Fundamental Research Grant Scheme (FRGS): FP005-2017A by Ministry of Higher Education, Malaysia, University of Malaya Research Grant (UMRG-RP021C-13HTM)
Uncontrolled Keywords: Viruses; Infection; Zika virus
Subjects: Q Science > QR Microbiology
R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 22 Mar 2020 10:27
Last Modified: 22 Mar 2020 10:27
URI: http://eprints.um.edu.my/id/eprint/24084

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