Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane

Ng, I-Son and Tang, Malcolm Siong Yii and Show, Pau Loke and Chiou, Zih Ming and Tsai, Jung Chin and Chang, Yu Kaung (2019) Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane. International Journal of Biological Macromolecules, 132. pp. 615-628. ISSN 0141-8130, DOI https://doi.org/10.1016/j.ijbiomac.2019.03.235.

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Official URL: https://doi.org/10.1016/j.ijbiomac.2019.03.235


In this work, a chitosan-modified nanofiber membrane was fabricated and used to examine the permeation characteristics of C-phycocyanin (CPC) obtained from Spirulina platensis. The effects of NaCl concentration (0.1–1.0 M), chitosan coupling pH (6–8), chitosan coupling concentration (0.1–3.0%), algal solution pH (6–8), algal mass concentration (0.1–1.0% dw/v), and membrane flux (4.08 × 10 −2 –2.04 × 10 −1 mL/min·cm 2 ) on the penetration performance of the membrane for CPC were investigated. The results show that the order of binding selectivity of the membrane for these proteins is contaminating proteins (TP) > allophycocyanin (APC) > CPC. TP and APC molecules were more easily adsorbed by the chitosan-modified membrane, and the CPC molecules most easily penetrated the membrane without being adsorbed, enhancing CPC purity. The purification factor and total mass flux were 3.3 fold and 66%, respectively, in a single step. © 2019 Elsevier B.V.

Item Type: Article
Funders: Ministry of Science and Technology, Taiwan (grant numbers MOST 105-2622-E-131-004-CC3, 104-2221-E-131-021, and 103-2221-E-131-023)
Additional Information: Doctoral Degree (phd). Malcolm Tang Siong Yii.
Uncontrolled Keywords: Chitosan-modified nanofiber membrane; Purity; C-phycocyanin; Spirulina platensis; Negative chromatography
Subjects: Q Science > QC Physics
Q Science > QR Microbiology
T Technology > TP Chemical technology
Divisions: Faculty of Science > Department of Physics
Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 20 Feb 2020 00:59
Last Modified: 20 Feb 2020 00:59
URI: http://eprints.um.edu.my/id/eprint/23849

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