Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum

Quah, Yixian and Mohd Ismail, Nor Ismaliza and Ooi, Jillian Lean Sim and Affendi, Yang Amri and Abd Manan, Fazilah and Teh, Lai Kuan and Wong, Fai Chu and Chai, Tsun Thai (2019) Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum. Journal of Zhejiang University-SCIENCE B, 20 (1). pp. 59-70. ISSN 1673-1581, DOI https://doi.org/10.1631/jzus.B1700586.

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Official URL: https://doi.org/10.1631/jzus.B1700586


Globally, peptide-based anticancer therapies have drawn much attention. Marine organisms are a reservoir of anticancer peptides that await discovery. In this study, we aimed to identify cytotoxic oligopeptides from Sarcophyton glaucum. Peptides were purified from among the S. glaucum hydrolysates produced by alcalase, chymotrypsin, papain, and trypsin, guided by a methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay on the human cervical cancer (HeLa) cell line for cytotoxicity evaluation. Purification techniques adopted were membrane ultrafiltration, gel filtration chromatography, solid phase extraction (SPE), and reversed-phase high-performance liquid chromatography (RP-HPLC). Purified peptides were identified by de novo peptide sequencing. From papain hydrolysate, three peptide sequences were identified: AGAPGG, AERQ, and RDTQ (428.45, 502.53, and 518.53 Da, respectively). Peptides synthesized from these sequences exhibited cytotoxicity on HeLa cells with median effect concentration (EC 50 ) values of 8.6, 4.9, and 5.6 mmol/L, respectively, up to 5.8-fold stronger than the anticancer drug 5-fluorouracil. When tested at their respective EC 50 , AGAPGG, AERQ, and RDTQ showed only 16%, 25%, and 11% cytotoxicity to non-cancerous Hek293 cells, respectively. In conclusion, AERQ, AGAPGG, and RDTQ are promising candidates for future development as peptide-based anticancer drugs. © 2019, Zhejiang University and Springer-Verlag GmbH Germany, part of Springer Nature.

Item Type: Article
Funders: Fundamental Research Grant Scheme of the Ministry of Higher Education, Malaysia (FRGS/1/2013/ST04/UTAR/ 02/1)
Uncontrolled Keywords: Anticancer therapy; Bioactive peptide; Cytotoxicity; HeLa cells; Q516; Sarcophyton glaucum; Soft coral
Subjects: G Geography. Anthropology. Recreation
Q Science > QR Microbiology
R Medicine
S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Faculty of Arts and Social Sciences
Faculty of Science > Institute of Biological Sciences
Deputy Vice Chancellor (Research & Innovation) Office > Institute of Ocean and Earth Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 10 Feb 2020 08:10
Last Modified: 10 Feb 2020 08:10
URI: http://eprints.um.edu.my/id/eprint/23723

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