Detection of human sarcocystosis using dried blood on filter papers: An immunofluorescent antibody test

Sharudin, P.S. and Rahimi, Razuin and Latif, Baha and Kutty, Methil Kannan and Muslim, Azdayanti and Abdul Rahim, A.S. and Abdul Aziz, M. and Mahfudz, Anis Shafina and Lim, Yvonne Ai Lian and Heo, Chong Chin and Md Noor, Shahidan and Hiu, J. and Hussaini, Jamal (2019) Detection of human sarcocystosis using dried blood on filter papers: An immunofluorescent antibody test. Tropical Biomedicine, 36 (2). pp. 453-458. ISSN 0127-5720,

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Sarcocystosis, a parasitic infection caused by a protozoa belonging to the genus Sarcocystis, is found worldwide in both and animals. Sarcocystis spp., require two animal hosts to complete their life cycle. The infection has gathered more global attention after recent outbreaks, especially amongst wester travellers to Malaysia. Other than sporadic cases and the current outbreaks, little information is available regarding human Sarcocystis infection in Malaysia. The present study aims to determine the prevalence of sarcocystosis among humans using an immunofluorescent antibody (IFA) test applied to dried blood on filter papers. A total of 200 blood samples were collected on filter papers from autopsy cases at two Malaysian hospitals: Sungai Buloh Hospital (peninsular Malaysia) and Queen Elizabeth Hospital (Malaysian Borneo). Antigens were prepared from bradyzoites harvested from positive goats’ muscle samples. Of the 200 samples, 32 (16%) had Sarcocystis antibodies that showed positive fluorescence reactions on filter papers. There was no significant difference (t-test, p value > 0.05) in prevalence rates between samples collected from autopsies at peninsular Malaysia and Malaysian Borneo. The results demonstrated that the filter paper technique can be used as one of the alternative serological tests in the diagnostic of human sarcocystosis. © 2019, Malaysian Society for Parasitology. All rights reserved.

Item Type: Article
Funders: Ministry of Education (MOE), Malaysia under the Research Acculturation Grant Scheme (RAGS): Grant number [600-RMI/RAGS 5/3 (76/2014)]
Uncontrolled Keywords: Sarcocystis; Sarcocystosis; Sarcocyst wall
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 20 Jan 2020 01:11
Last Modified: 20 Jan 2020 01:11

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