Investigation of possible rickettsial infection in patients with malaria

Tay, Sun Tee and Kho, Kai Ling and Vythilingam, Indra and Ooi, Choo Huck and Lau, Yee Ling (2019) Investigation of possible rickettsial infection in patients with malaria. Tropical Biomedicine, 36 (1). pp. 257-262. ISSN 0127-5720,

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Rickettsioses are a common health problem in many geographical areas, including rural areas in Southeast Asia. Co-infection of rickettsioses and malaria has been reported in Africa, where common reservoir and vectors are available. In this study, blood samples of Malaysian patients microscopically positive (n=148) and negative (n=88) for malaria parasites (Plasmodium knowlesi, Plasmodium malariae, Plasmodium falciparum, and Plasmodium vivax) were screened for the presence of rickettsial DNA, using PCR assays targeting specific genes. A partial fragment of rickettsial ompB gene was successfully amplified and sequenced from a patient microscopically positive for Plasmodium spp. and PCR-positive for P. vivax. BLAST analysis of the ompB sequence demonstrated the highest sequence similarity (99.7% similarity, 408/409nt) with Rickettsia sp. RF2125 (Genbank accession no. JX183538) and 91.4% (374/409 nt) similarity with Rickettsia felis URRWXCal2 (Genbank accession no. CP000053). This study reports rickettsial infection in a malaria patient for the first time in the Southeast Asia region. © 2019, Malaysian Society for Parasitology. All rights reserved.

Item Type: Article
Funders: Ministry of Science, Technology and Innovation E-Science Fund (SF014-2015), High Impact Research Chancellery
Uncontrolled Keywords: Rickettsia; Ticks; SFG rickettsiae
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 23 Dec 2019 02:00
Last Modified: 23 Dec 2019 02:00

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