Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum

Wu, Songcui and Gu, Wenhui and Huang, Aiyou and Li, Yuanxiang and Kumar, Manoj and Lim, Phaik Eem and Huan, Li and Gao, Shan and Wang, Guangce (2019) Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum. Microbial Cell Factories, 18 (1). p. 161. ISSN 1475-2859, DOI https://doi.org/10.1186/s12934-019-1214-x.

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Official URL: https://doi.org/10.1186/s12934-019-1214-x


Background: Numerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a decrease of growth in the majority of microalgae. We previously found that elevated CO2 concentration increased lipid productivity as well as growth in Phaeodactylum tricornutum, along with an enhancement of the oxidative pentose phosphate pathway (OPPP) activity. The purpose of this work directed toward the verification of the critical role of glucose-6-phosphate dehydrogenase (G6PDH), the rate-limiting enzyme in the OPPP, in lipid accumulation in P.Tricornutum and its simultaneous rapid growth rate under high-CO2 (0.15%) cultivation. Results: In this study, G6PDH was identified as a target for algal strain improvement, wherein G6PDH gene was successfully overexpressed and antisense knockdown in P.Tricornutum, and systematic comparisons of the photosynthesis performance, algal growth, lipid content, fatty acid profiles, NADPH production, G6PDH activity and transcriptional abundance were performed. The results showed that, due to the enhanced G6PDH activity, transcriptional abundance and NAPDH production, overexpression of G6PDH accompanied by high-CO2 cultivation resulted in a much higher of both lipid content and growth in P.Tricornutum, while knockdown of G6PDH greatly decreased algal growth as well as lipid accumulation. In addition, the total proportions of saturated and unsaturated fatty acid, especially the polyunsaturated fatty acid eicosapentaenoic acid (EPA; C20:5, n-3), were highly increased in high-CO2 cultivated G6PDH overexpressed strains. Conclusions: The successful of overexpression and antisense knockdown of G6PDH well demonstrated the positive influence of G6PDH on algal growth and lipid accumulation in P.Tricornutum. The improvement of algal growth, lipid content as well as polyunsaturated fatty acids in high-CO2 cultivated G6PDH overexpressed P.Tricornutum suggested this G6PDH overexpression-high CO2 cultivation pattern provides an efficient and economical route for algal strain improvement to develop algal-based biodiesel production. © 2019 The Author(s).

Item Type: Article
Funders: National Natural Science Foundation of China (41806171 and 41876158), National Key R&D Program of China (2018YFD0901501-1), Haikou Marine Economic Demonstration City Industrial Chain Collaborative Innovation Project (HHCL201811), Tianjin Marine Economic Demonstration (BHSF2017-21)
Uncontrolled Keywords: Algal growth rate; Antisense knockdown; CO2; Glucose-6-phosphate dehydrogenase; Lipid accumulation; Overexpression; Phaeodactylum tricornutum
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Deputy Vice Chancellor (Research & Innovation) Office > Institute of Ocean and Earth Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 28 Nov 2019 02:26
Last Modified: 28 Nov 2019 02:26
URI: http://eprints.um.edu.my/id/eprint/23144

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