Analysis of ESTs generated from inner bark tissue of an Acacia auriculiformis x Acacia mangium hybrid

Yong, Christina Seok Yien and Choong, Chee Yen and Cheong, P.L. and Pang, Sze Lei and Nor Amalina, R. and Harikrishna, Jennifer Ann and Mat-Isa, Mohd Noor and Hedley, P. and Milne, Linda and Vaillancourt, R. and Wickneswari, R. (2011) Analysis of ESTs generated from inner bark tissue of an Acacia auriculiformis x Acacia mangium hybrid. Tree Genetics & Genomes, 7 (1). pp. 143-152. ISSN 1614-2942, DOI

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The majority of plant expressed sequence tags (ESTs) available in the public databases are from non-woody species such as Arabidopsis, maize, soybean, and rice, with the exception of the model tree species, Populus. In this study, we report the first EST database constructed from the commercially important tree species Acacia. ESTs were generated from pooled RNA extracted from the mature, intermediate, and young inner bark tissues of an Acacia auriculiformis x Acacia mangium hybrid. Finally, 3,182 high-quality ESTs were analyzed representing a total of 1,982 unique transcripts (663 contigs and 1,319 singletons) for the inner bark cDNA library. A total of 1,053 unigenes (1,750 ESTs) showed similarities with protein sequences in public database where 867 were significant matches (E value ≤ e-10). Further analysis performed on unigenes with significant matches and known functions identified 231 genes including genes involved in cellulose, lignin, and hemicellulose biosynthesis. For cDNA library validation, quantitative real-time RT-PCR was performed to study the expression levels of the cellulose and lignin genes identified from this EST database in the phloem tissue of A. mangium, A. auriculiformis, and A. auriculiformis x A. mangium hybrid. All the seven candidate genes were expressed in all the individuals studied confirming the dependability of the EST database. The cDNA library and the EST database constructed are valuable resources for forest tree research aiming towards understanding the genetic control of wood formation and in the future endeavors to modify wood and fiber profile through marker-assisted breeding programs. © 2010 Springer-Verlag.

Item Type: Article
Funders: Priority Research Grant under the Intensified Research Project Area Scheme to Universiti Kebangsaan Malaysia from the Ministry of Science, Technology & Innovation, Malaysia
Uncontrolled Keywords: cDNA library; Comparative sequence analysis; Library validation; Transcript profiling; Wood formation genes
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 18 Nov 2019 01:32
Last Modified: 18 Nov 2019 01:32

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