Metabolic and physiological regulation of Chlorella sp. (Trebouxiophyceae, Chlorophyta) under nitrogen deprivation

Yong, Wai Kuan and Lim, Phaik Eem and Vello, Vejeysri and Sim, Kae Shin and Majid, Nazia Abdul and Mustafa, Emienour Muzalina and Sulaiman, Nik Meriam Nik and Liew, Kan Ern and Chen, Brenna Jia Tian and Phang, Siew Moi (2019) Metabolic and physiological regulation of Chlorella sp. (Trebouxiophyceae, Chlorophyta) under nitrogen deprivation. Journal of Oceanology and Limnology, 37 (1). pp. 186-198. ISSN 2096-5508, DOI

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A freshwater green microalgae Chlorella sp., UMACC344 was shown to produce high lipid content and has the potential to be used as feedstock for biofuel production. In this study, photosynthetic efficiency, biochemical profiles and non-targeted metabolic profiling were studied to compare between the nitrogen-replete and deplete conditions. Slowed growth, change in photosynthetic pigments and lowered photosynthetic efficiency were observed in response to nitrogen deprivation. Biochemical profiles of the cultures showed an increased level of carbohydrate, lipids and total fatty acids, while the total soluble protein content was lowered. A trend of fatty acid saturation was observed in the nitrogen-deplete culture with an increase in the level of saturated fatty acids especially C16:0 and C18:0, accompanied by a decrease in proportions of monounsaturated and polyunsaturated fatty acids. Fifty-nine metabolites, including amino acids, lipids, phytochemical compounds, vitamins and cofactors were significantly dysregulated and annotated in this study. Pathway mapping analysis revealed a rewiring of metabolic pathways in the cells, particularly purine, carotenoid, nicotinate and nicotinamide, and amino acid metabolisms. Within the treatment period of nitrogen deprivation, the key processes involved were reshuffling of nitrogen from proteins and photosynthetic machinery, together with carbon repartitioning in carbohydrates and lipids.

Item Type: Article
Funders: Aerospace Malaysia Innovation Centre & Airbus Group Innovation (No. PV001-2013), Ministry of Higher Education Malaysia HICoE grant (No. IOES-2014H), Fundamental Research Grant Scheme (No. FP048-2016), University of Malaya UMCoE RU Grant (No. RU009H-2015)
Uncontrolled Keywords: Chlorella sp; fatty acid; lipid; metabolic profiling; nitrogen stress
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
T Technology > TP Chemical technology
Divisions: Faculty of Engineering
Faculty of Science > Institute of Biological Sciences
Deputy Vice Chancellor (Research & Innovation) Office > Institute of Ocean and Earth Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 04 Nov 2019 09:04
Last Modified: 04 Nov 2019 09:04

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