Antioxidant assay of Alstonia angustifolia ethanolic leaf extract

Ab Rahim, Nurhidayah and Zakaria, Noorzafiza and Dzulkarnain, Syarifah Masyitah Habib and Azahar, Nazar Mohd Zabadi Mohd and Abdulla, Mahmood Ameen (2018) Antioxidant assay of Alstonia angustifolia ethanolic leaf extract. Journal of Advanced Research in Fluid Mechanics and Thermal Sciences, 42 (1). pp. 80-86. ISSN 2289-7879,

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In current study, the ability of the ethanolic extract of Alstonia angustifolia in scavenging free radicals was assessed by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and hydrogen peroxide (H2O2) radical scavenging assay. The results suggested that the ethanolic extract of A. angustifolia leaves has a notable antioxidant activity. In FRAP assay, it showed that the extract have higher total antioxidant activity with FRAP value is 1868.33 μM/g Fe (ii) dry mass ± 0.15 than the control, quercetin with FRAP value is 1336.9 μM/g Fe (II) dry mass ± 0.12 and ascorbic acid with FRAP value is 1720 μM/g Fe (II) dry mass ± 0.02. For DPPH assay, the IC50 value of the extract is 384.77 while the IC50 value of standards of ascorbic acid and quercetin are 18.07 μg/ml and 39.60 μg/ml, respectively. For H2O2 scavenging assay, the IC50 value for the extract was discovered to be 186.77 μg/ml compared to standard ascorbic acid 466.56 μg/ml. Thus, the study suggests that A. angustifolia ethanolic leaf extract has a good origin of natural antioxidants and might be beneficial in impeding the oxidative stress progression thus averting diseases that related to free radicals.

Item Type: Article
Uncontrolled Keywords: Alstonia angustifolia; antioxidant assay; DPPH; FRAP; Hydrogen peroxide scavenging assay
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 21 Oct 2019 01:27
Last Modified: 21 Oct 2019 01:27

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