Efficient micropropagation protocol and genome size estimation of an important cover crop, Mucuna bracteata DC. ex Kurz

Abd Aziz, Nazrin and Tan, Boon Chin and Othman, Rofina Yasmin and Khalid, Norzulaani (2018) Efficient micropropagation protocol and genome size estimation of an important cover crop, Mucuna bracteata DC. ex Kurz. Plant Cell, Tissue and Organ Culture, 132 (2). pp. 267-278. ISSN 0167-6857, DOI https://doi.org/10.1007/s11240-017-1376-3.

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Official URL: https://doi.org/10.1007/s11240-017-1376-3


Mucuna bracteata DC. ex Kurz is an important cover crop in plantations across the tropics. However, low germination rate and poor viability of Mucuna seeds pose significant challenges of using the seeds as starting material. To address these limitations, we have optimized seed germination conditions (such as scarification period, surface sterilization protocols and imbibition period) and in vitro propagation protocols for M. bracteata. We found that seeds treated with sulphuric acid for 30 min, imbibed for 6 h and incubated in dark conditions on a wet cotton roll (10 mL of sterile distilled water) supplemented with 0.1% activated charcoal produced the highest percentage of seed germination (44%) and seed vigor index. In vitro-derived cotyledonary nodes showed the highest number of shoots per explant (5.60) and rooting response (92.9%) when cultured on Murashige and Skoog medium containing 4.44 µM 6-benzylaminopurine and 10.7 µM 1-naphthaleneacetic acid, respectively. Of the 100 rooted plantlets acclimatized, 89.0% survived after 4 weeks of transplanting. Single sequence repeat and flow cytometry analysis were performed to confirm the genetic fidelity of the plants. Our protocol offers, for the first time, a simple and effective seed germination and scalable propagation procedures for M. bracteata. Furthermore, we have also estimated the genome size (1448 ± 9 Mb) and DNA content (1.48 ± 0.01 pg) for M. bracteata that can be used for future cytogenetic studies on genetic diversity and gene exchange.

Item Type: Article
Funders: University of Malaya Bright Sparks Programme (BSP/APP/2022/2014), University of Malaya PPP Grant (Grant No: PG254-2016A), High Impact Research Programme of University of Malaya, Malaysia (UM.C/625/1/HIR/MOHE/SCI/18), CEBAR RU (RU006-2017)
Uncontrolled Keywords: Genome size; Hydropriming; Leguminous plants; Osmopriming; Plant propagation; Regeneration; Seed germination
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 21 Oct 2019 00:54
Last Modified: 21 Oct 2019 00:54
URI: http://eprints.um.edu.my/id/eprint/22758

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