Isolation of Bacillus cereus from botanical soil and subsequent biodegradation of waste engine oil

Bhurgri, Shazia and Talpur, Farah Naz and Nizamani, Shafi Muhammad and Afridi, Hassan Imran and Surhio, Muhammad Ali and Shah, Muhammad Raza and Bong, Chui Wei (2018) Isolation of Bacillus cereus from botanical soil and subsequent biodegradation of waste engine oil. International Journal of Environmental Science and Technology, 15 (7). pp. 1453-1466. ISSN 1735-1472, DOI

Full text not available from this repository.
Official URL:


Waste engine oil causes a vital environmental pollution when it spill during change and transportation and products of waste engine oil causes lethal effects to the living systems. Thus, abiotic and biotic approaches are being extensively used for removal of waste engine oil pollution. Therefore in present study, waste engine oil degradation was accomplished by a new bacterial culture, isolated from the soil by an enrichment technique. Morphological, biochemical and gene sequence analysis revealed that isolate was Bacillus cereus. Subsequently, biodegradation potential of B. cereus for waste engine oil was studied. Experimental variables, such as pH, substrate concentration, inoculum size, temperature and time on the biodegradation, were checked in mineral salt medium. The biodegradation efficiency of B. cereus was determined by gravimetry, UV–visible spectrophotometry and gas chromatography. In addition, waste engine oil was also characterized by GC–MS and FTIR for its major constituents, which showed total 38 components in waste engine oil, including hopanes, benzopyrene, long-chain aliphatic hydrocarbons, dibenzothiophenes, biphenyl and their derivatives. Results of successive biodegradation indicated that B. cereus was capable to degrade 1% of waste engine oil with 98.6% degradation potential at pH 7 within 20 days. Hence, B. cereus presents an innovative tool for removing the engine oil from the contaminated area.

Item Type: Article
Uncontrolled Keywords: Bacillus cereus; Biodegradation; Engine oil contamination; Mineral Salt Medium; Oil spill; Waste engine oil
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 13 Sep 2019 01:26
Last Modified: 13 Sep 2019 01:26

Actions (login required)

View Item View Item