Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis

Eamsobhana, Praphathip and Tungtrongchitr, Anchalee and Wanachiwanawin, Darawan and Yong, Hoi Sen (2018) Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis. International Journal of Infectious Diseases, 73. pp. 69-71. ISSN 1201-9712, DOI

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Objectives: The serological diagnosis of human infection with Angiostrongylus cantonensis remains problematic because there are no commercially available validated tests. Most laboratories use domestically prepared tests such as the enzyme-linked immunosorbent assay (ELISA) or immunoblotting. Since laboratory facilities are not always available in endemic areas, we developed and assessed a rapid lateral flow immunochromatographic assay (AcQuick Dx Test) to detect anti-A. cantonensis antibodies in human serum. Methods: The test device was assembled with purified 31-kDa glycoprotein as diagnostic antigen and with gold-labelled anti-human immunoglublin-G as the detector reagent. A total of 97 serum samples were tested – 19 samples from clinically diagnosed patients with detectable A. cantonensis-specific antibody in immunoblotting; 43 samples from patients with other parasitic diseases, i.e. gnathostomiasis (n = 13), toxocariasis (n = 2), trichinellosis (n = 2), hookworm infection (n = 4), filariasis (n = 5), cysticercosis (n = 9), paragonimiasis (n = 2), opisthorchiasis (n = 3), and malaria (n = 3); and 35 samples from normal healthy subjects. Results: The sensitivity, specificity, positive predictive value and negative predictive value of AcQuick Dx Test to detect anti-A. cantonensis specific antibodies in serologically confirmed angiostrongyliasis cases, were 100%, 98.72%, 95% and 100%, respectively. Positive AcQuick Dx was observed in 1 of 4 cases with hookworm infections. No positive AcQuick Dx was observed in cases with other parasitic diseases, and the individual healthy subjects. Conclusions: AcQuick Dx Test is rapid, highly sensitive and specific, and easy to perform without additional equipment or ancillary supplies. It yields results that are interpreted visually, and possesses a long shelf-life at room temperature. Thus, it can be applied as an additional test for clinical diagnostic support of angiostrongyliasis either in conventional laboratories or for remote areas where laboratory infrastructure is not available.

Item Type: Article
Funders: Department of Disease Control, Ministry of Public Health, Thailand, University of Malaya (YHS: grants H-5620009)
Uncontrolled Keywords: Angiostrongylus cantonensis; Lateral flow immunochromatographic assay; Rapid test; 31-kDa antigen; Immunodiagnosis
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 30 Aug 2019 01:25
Last Modified: 04 Dec 2019 08:50

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