Molecular Diagnosis of Microsporidia among Immunocompromised Patients in Kuala Lumpur, Malaysia

Hassan, Nur-Amirah and Lim, Yvonne Ai Lian and Mahmud, Rohela and Mohd-Shaharuddin, Norashikin and Wan Sulaiman, Wan Yusoff and Ngui, Romano (2018) Molecular Diagnosis of Microsporidia among Immunocompromised Patients in Kuala Lumpur, Malaysia. The American Journal of Tropical Medicine and Hygiene, 99 (6). pp. 1562-1566. ISSN 0002-9637, DOI

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Microsporidia are obligate intracellular parasitic fungi causing chronic diarrhea, particularly among immunocompromised patients. The current method used for diagnosis is based on conventional microscopy, which does not differentiate parasites at the species level. The present study was carried out to identify microsporidian species in immunocompromised patients. From March 2016 to March 2017, a total of 289 archived stool samples were examined microscopically for microsporidian spores using Gram-chromotrope Kinyoun (GCK) stain. Positive stool samples by microscopy were subjected to polymerase chain reaction and sequencing for species identification. Based on microscopy examination, the overall prevalence of microsporidian infections was 32.2% (93/289; 95% CI = 27.1–37.8). Of these stool samples, 45 samples were successfully amplified and confirmed as Enterocytozoon bieneusi. No Encephalitozoon intestinalis was detected. Accurate identification of species might help clinicians to decide appropriate management strategies as dissemination risks and treatment response vary for different species, hence improving the management of microsporidian infections.

Item Type: Article
Funders: University of Malaya (RP050B-17HTM and BKS005-2017)
Uncontrolled Keywords: adolescent; adult; aged; child; controlled study; diagnostic accuracy; Enterocytozoon bieneusi; feces analysis; Federal Territory of Kuala Lumpur; female; fungus identification; health care management; health care personnel; human; immunocompromised patient; major clinical study; male; microscopy; Microsporidia; microsporidiosis; molecular diagnosis; polymerase chain reaction; prevalence; risk factor; treatment response
Subjects: R Medicine
Divisions: Faculty of Medicine
Centre of Excellence for Research in AIDS (CERiA)
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 08 Aug 2019 06:53
Last Modified: 08 Aug 2019 06:53

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