18s rDNA Sequence Analysis of Microfungi from Biofloc-based System in Pacific Whiteleg Shrimp, Litopenaeus vannamei Culture

Kasan, Nor Azman and Ghazali, Nurarina Ayuni and Hashim, Nurul Fakriah Che and Jauhari, Iswadi and Jusoh, Ahmad and Ikhwanuddi, Mhd (2018) 18s rDNA Sequence Analysis of Microfungi from Biofloc-based System in Pacific Whiteleg Shrimp, Litopenaeus vannamei Culture. Biotechnology, 17 (3). pp. 135-141. ISSN 1682-296X, DOI https://doi.org/10.3923/biotech.2018.135.141.

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Official URL: https://doi.org/10.3923/biotech.2018.135.141


Background and Objective: One of the major problem in Pacific Whiteleg shrimp, Litopenaeus vannamei farming industry is the accumulation of excess toxicant such as ammonia and nitrite. Biofloc technology served as an alternative solution to solve this problem by recycling the water for sustainable aquaculture management due to water scarcity, bio-security and bio-economy. Biological communities within biofloc are mainly composed of micro-organisms which plays an important role in biodegradation of organic material and removal of toxic contaminants. For the purpose of this study, molecular identification of microfungi isolated from biofloc was conducted. Materials and Methods: Isolation of microfungi composition was conducted by culturing the biofloc samples onto Potato Dextrose Agar (PDA) media which further identified molecularly using 18s rDNA sequences analysis. Results: The identified microfungi includes genus from Aspergillus versicolor, A. Niger, A. tamarii, A. flavipes, A. aculeatus, Penicillium citrinum, P. griseofulvum, Trichoderma virens and Pestalotiopsis microspora. The most dominant microfungi were genus Aspergillus sp. followed by the presence of Penicillium sp. Trichoderma sp. and Pestalotiopsis sp. Conclusion: Existence of microfungi species such as Aspergillus sp. and Penicillium sp. in biofloc were perfect candidate in forming biofloc that could reduce water pollution towards a sustainable aquaculture industry.

Item Type: Article
Funders: Fundamental Research Grant Scheme, FRGS (vot No: 59401), Ministry of Education, Malaysia
Uncontrolled Keywords: 18s rDNA; Bio-economy; Bio-security; Biofloc technology; L. vannamei; Microfungi
Subjects: T Technology > TJ Mechanical engineering and machinery
Divisions: Faculty of Engineering
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 19 Jul 2019 09:17
Last Modified: 19 Jul 2019 09:17
URI: http://eprints.um.edu.my/id/eprint/21673

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