Immobilization of Bovine Serum Albumin on the Chitosan/PVA Film

Khairuddin, Nurul Mujahidah Ahmad and Afifi, Amalina Muhammad and Hashim, Nur Awanis and Mohamad, Shaza Eva and Kalantari, Katayoon (2018) Immobilization of Bovine Serum Albumin on the Chitosan/PVA Film. Sains Malaysiana, 47 (6). pp. 1311-1318. ISSN 0126-6039, DOI

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Chitosan/polyvinyl alcohol (Chitosan/PVA) blended film was prepared by direct blend process and solution casting methods. In order to reduce the swelling ratio and enhance the chemical and mechanical stability, Chitosan/PVA film was crosslinked with glutaraldehyde in order to produce Chitosan-g-PVA. Bovine serum albumin (BSA) was used as a model protein to incorporate into the Chitosan-g-PVA. The chemical structure and morphological characteristics of films were studied by FT-IR and scanning electron microscopy (SEM). Mechanical and physical properties of blended films such as tensile properties in the dry and wet states, water uptake and water contact angle measurement were characterized. Blending PVA and chitosan improved strength and flexibility of the films. Crosslinking with glutaraldehyde further improves the tensile strength and decrease the hydrophilicity of films. BSA immobilized on the Chitosan-g-PVA film was calculated as BSA encapsulation efficiency.

Item Type: Article
Funders: University of Malaya Research (Grant RP034A-15AET) and Fundamental Research Grant Scheme (FRGS-FP026-2014B)
Uncontrolled Keywords: Bovine serum albumin; Chitosan; Glutaraldehyde; Polyvinyl alcohol
Subjects: T Technology > TJ Mechanical engineering and machinery
T Technology > TP Chemical technology
Divisions: Faculty of Engineering
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 18 Jul 2019 07:19
Last Modified: 18 Jul 2019 07:19

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