Microbial diversity of thermophiles with biomass deconstruction potential in a foliage‐rich hot spring

Lee, Li Sin and Goh, Kian Mau and Chan, Chia Sing and Tan, Geok Yuan Annie and Yin, Wai Fong and Chong, Chun Shiong and Chan, Kok Gan (2018) Microbial diversity of thermophiles with biomass deconstruction potential in a foliage‐rich hot spring. MicrobiologyOpen, 7 (6). e00615. ISSN 2045-8827, DOI https://doi.org/10.1002/mbo3.615.

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Official URL: https://doi.org/10.1002/mbo3.615


The ability of thermophilic microorganisms and their enzymes to decompose biomass have attracted attention due to their quick reaction time, thermostability, and decreased risk of contamination. Exploitation of efficient thermostable glycoside hydrolases (GHs) could accelerate the industrialization of biofuels and biochemicals. However, the full spectrum of thermophiles and their enzymes that are important for biomass degradation at high temperatures have not yet been thoroughly studied. We examined a Malaysian Y-shaped Sungai Klah hot spring located within a wooded area. The fallen foliage that formed a thick layer of biomass bed under the heated water of the Y-shaped Sungai Klah hot spring was an ideal environment for the discovery and analysis of microbial biomass decay communities. We sequenced the hypervariable regions of bacterial and archaeal 16S rRNA genes using total community DNA extracted from the hot spring. Data suggested that 25 phyla, 58 classes, 110 orders, 171 families, and 328 genera inhabited this hot spring. Among the detected genera, members of Acidimicrobium, Aeropyrum, Caldilinea, Caldisphaera, Chloracidobacterium, Chloroflexus, Desulfurobacterium, Fervidobacterium, Geobacillus, Meiothermus, Melioribacter, Methanothermococcus, Methanotorris, Roseiflexus, Thermoanaerobacter, Thermoanaerobacterium, Thermoanaerobaculum, and Thermosipho were the main thermophiles containing various GHs that play an important role in cellulose and hemicellulose breakdown. Collectively, the results suggest that the microbial community in this hot spring represents a good source for isolating efficient biomass degrading thermophiles and thermozymes.

Item Type: Article
Funders: Postgraduate Research Fund grant, Grant/ Award Number: PG124-2016A, University of Malaya, Grant/Award Number: GA001-2016 and GA002-2016, Universiti Teknologi Malaysia GUP, Grant/Award Number: 09H98, 16H89 and 4B297
Uncontrolled Keywords: Biofilm; biofuel; biomass degradation; cellulase; hot spring; thermophile
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 20 Jun 2019 04:42
Last Modified: 20 Jun 2019 04:42
URI: http://eprints.um.edu.my/id/eprint/21513

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