UV-B irradiation effects on pigments and cytological behaviour of callus in sainfoin (Onobrychis viciifolia Scop.)

Mohajer, Sadegh and Taha, Rosna Mat and Mohajer, Minoo and Anuar, Nordiyanah (2018) UV-B irradiation effects on pigments and cytological behaviour of callus in sainfoin (Onobrychis viciifolia Scop.). Pigment & Resin Technology, 47 (6). pp. 496-501. ISSN 0369-9420, DOI https://doi.org/10.1108/PRT-11-2016-0102.

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Official URL: https://doi.org/10.1108/PRT-11-2016-0102


Purpose: This study aims to determine the appropriate irradiation dose for induction of somaclonal variation in mass of unorganized parenchyma cells derived from sainfoin (Onobrychis viciifolia) tissues. Design/methodology/approach: To investigate the feasibility of UV-B irradiation (312 nm), seeds and callus of sainfoin were exposed to five different intensities (20-100 per cent) for evaluating the effectiveness of phytochemical constituents and cellular behaviour. Findings: Although percentage of seed viability reduced in 20 per cent intensity of UV-B irradiation compared with control seeds, the germination speed significantly enhanced from 3.58 to 5.42. The spectrophotometer analysis confirmed that concentrations of chlorophyll (a and b) decreased after UV-B exposure as compared with control callus. Also, carotenoid content showed significant differences among treated calli. Flavonoid and phenolic contents were observed to gradually increase when the non-irradiated callus subjected to 40 and 60 per cent intensities of UV-B irradiation, respectively. Observation on cellular behaviour such as determination of nuclear and cell areas, mitotic index and chromosomal aberrations were also detected to change in different intensities of UV-B irradiation. Fragmented and aneuploidy aberrations were only observed in exposed cells with more than 40 per cent intensity of UV-B irradiation. Originality/value: In reality, radiocytological evaluation was proven to be essential in deducing the effectiveness of UV-B irradiation to induce somaclonal variation in callus tissue of sainfoin.

Item Type: Article
Uncontrolled Keywords: Pigment; Phenolic; Callus; Cytology; Flavonoid; UV-B irradiation
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 21 May 2019 06:32
Last Modified: 21 May 2019 06:32
URI: http://eprints.um.edu.my/id/eprint/21270

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