Antifungal and cytotoxic activities of extracts obtained from underutilised edible tropical fruits

Ong, Cheong Wei and Chan, Yik Sin and Khoo, Kong Soo and Ong, Hean Chooi and Sit, Nam Weng (2018) Antifungal and cytotoxic activities of extracts obtained from underutilised edible tropical fruits. Asian Pacific Journal of Tropical Biomedicine, 8 (6). pp. 313-319. ISSN 2221-1691, DOI

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Objective: To evaluate antifungal and cytotoxic activities of four underutilised fruit species, i.e. Artocarpus altilis (breadfruit), Cynometra cauliflora (nam-nam), Mangifera pajang (M. pajang) (Bambangan) and Physalis minima (wild gooseberry). Methods: Extracts from the fresh flesh of Artocarpus altilis and Cynometra cauliflora, the flesh and kernel of M. pajang, and the whole fruit of Physalis minima were obtained by sequential extraction using hexane, chloroform, ethyl acetate, ethanol, methanol and distilled water. Each extract was assessed against six species of human fungal pathogens using a colourimetric broth microdilution method. The cytotoxicity was evaluated using African monkey kidney epithelial (Vero) cells. Results: All 30 extracts showed inhibitory activity against Cryptococcus neoformans. However, none of the extracts were active against Aspergillus fumigatus. The ethanol, methanol and water extracts from the kernel of M. pajang fruit showed the strongest activity against three species of Candida and Trichophyton interdigitale, with a minimum inhibitory concentration range of 0.001 - 0.630 mg/mL. The corresponding mean 50% cytotoxic concentrations for these three extracts were 358.7, 158.4 and 261.3 μg/mL, respectively against Vero cells. In contrast, the flesh of M. pajang fruit (hexane, chloroform and ethyl acetate extracts) showed statistically significant (P<0.001; ANOVA) strong toxicity against the cells, with 30.6, 13.5 and 22.2 μg/mL of mean values of 50% cytotoxic concentrations, respectively. Conclusions: The results suggest that the bioactivity of the kernel of M. pajang fruit is more selective towards fungi and thus is a potential source of new antifungal agents.

Item Type: Article
Funders: Universiti Tunku Abdul Rahman Research Fund (IPSR/RMC/UTARRF/2012-C2/S03)
Uncontrolled Keywords: Artocarpus altilis; Cynometra cauliflora; Mangifera pajang; Physalis minima; Vero cell
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 25 Apr 2019 03:10
Last Modified: 25 Apr 2019 03:10

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