Characterization of fungi from Palm kernel cake (PKC) and the effect of storage temperature on fungi growth

Razali, S.M. and Lee, Hai Yen and Jinap, Selamat and Mahyudin, Nor Ainy (2018) Characterization of fungi from Palm kernel cake (PKC) and the effect of storage temperature on fungi growth. Pertanika Journal of Tropical Agricultural Science, 41 (1). pp. 115-128. ISSN 1511-3701,

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The widespread contamination of animal feed with mycotoxin is not a new issue worldwide. Apart from economic loss, mycotoxin can have adverse health effects on humans due to the carcinogenicity, teratogenicity and mutagenicity potential of the toxins. Palm kernel cake (PKC) is the largest animal feed production in Malaysia. PKC is a by-product of palm kernel oil processing and it has been exported as animal feed. The purpose of this study was to isolate and characterise toxigenic fungi cultured in three different media, Dichloran Rose Bengal Chloramphenicol (DRBC) Agar, Dichloran 18% Glycerol (DG18) Agar and Malt Extract Agar (MEA), derived from PKC that is stored under three different temperatures, 4°C, 25°C and 60°C. Identification of fungi was carried out based on macroscopy and microscopy as well as molecular identification. Four mycotoxigenic fungi were found: Aspergillus Niger, Aspergillus flavus, Aspergillus fumigatus and Penicilium citrinum. In order to characterise polymorphism of the isolates, RAPD assay was performed using OPA 3 as the primer. The software resulted in a constructed dendrogram that revealed the percentage of similarities between the typable isolates (A. fumigatus, A. Niger and P. citrinum) within range from 20% to 80%. The effect of storage temperature on the strains' enumeration is reported in this work. The distributing strains are influenced by the storage temperature of the PKC matrices. The findings clearly show that Aspergillus species profused at 25°C PKC storage, while it was restricted at low and high temperature.

Item Type: Article
Funders: Institure Tropical and Agricultute (LRGS 5526001), Faculty Food Science and Technology, University Putra Malaysia
Uncontrolled Keywords: Fungi; Media; Mycotoxin; PKC; RAPD assay; Temperature
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
R Medicine
Divisions: Deputy Vice Chancellor (Research & Innovation) Office > Tropical Infectious Diseases Research and Education Centre
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 15 Apr 2019 08:17
Last Modified: 15 Apr 2019 08:17

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