Sustainable approach in recycling of phase components of large scale aqueous two-phase flotation for lipase recovery

Sankaran, Revathy and Show, Pau Loke and Yap, Yee Jiun and Lam, Hon Loong and Ling, Tau Chuan and Pan, Guan Ting and Yang, Thomas C.K. (2018) Sustainable approach in recycling of phase components of large scale aqueous two-phase flotation for lipase recovery. Journal of Cleaner Production, 184. pp. 938-948. ISSN 0959-6526, DOI

Full text not available from this repository.
Official URL:


Recently, aqueous two-phase flotation system has become a powerful method for separation of biomolecules. However, the application of this technique for large scale operation elevates a concern on the environmental issue. This is due to the large amount of chemical consumption for phase components formation. Recycling of hydrophilic organic solvent and inorganic salt in aqueous two-phase flotation is an efficient and green technique for biomolecules recovery. Up to date, there is limited research concerning the recycling of the phase components. In this present work, recycling capability of pilot scale aqueous two-phase flotation composing alcohol/salt was employed to separate lipase from fermentation broth. The drive of this research is to economize the usage of chemicals, to reduce environmental pollution and to establish this approach for sustainable recovery of lipase. The aqueous two-phase flotation system investigated was composed of 1-propanol and ammonium sulphate whereby both phase components went through complete recycling process. Recycling of entire phase components is found to be a sustainable approach as it able to reduce the consumption of chemicals and it is an eco-friendly technique. From the result obtained, it was exhibited that by reusing the bottom phase, separation efficiency was sustained beyond 70%. As for the lipase yield, 80% of yield was achieved from recycling the crystallized salt that was recovered by dilution crystallization with methanol.

Item Type: Article
Funders: Fundamental Research Grant Scheme (Malaysia, FRGS/1/2015/SG05/UNIM/03/1), Ministry of Science and Technology, (MOSTI 02-02-12-SF0256), Prototype Research Grant Scheme (PRGS/2/2015/SG05/UNIM/03/1), SATU Joint Research Scheme (RU022E-2014 & RU018-2015)
Uncontrolled Keywords: Alcohol/salt; Aqueous two-phase flotation; Lipase; Phase components; Recycling
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 22 Mar 2019 01:57
Last Modified: 22 Mar 2019 01:57

Actions (login required)

View Item View Item