Purification, characterization, mode of action, and enhanced production of Salivaricin mmaye1, a novel bacteriocin from Lactobacillus salivarius SPW1 of human gut origin

Wayah, Samson Baranzan and Philip, Koshy (2018) Purification, characterization, mode of action, and enhanced production of Salivaricin mmaye1, a novel bacteriocin from Lactobacillus salivarius SPW1 of human gut origin. Electronic Journal of Biotechnology, 35. pp. 39-47. ISSN 0717-3458, DOI https://doi.org/10.1016/j.ejbt.2018.08.003.

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Official URL: https://doi.org/10.1016/j.ejbt.2018.08.003


Background: Emergence of antibiotic resistance among pathogenic and food spoilage bacteria such as Staphylococcus aureus, Micrococcus luteus, Streptococcus pyogenes, Streptococcus sanguinis, Streptococcus mutans, Bacillus cereus, and Listeria monocytogenes triggered the search for alternative antimicrobials. An investigation aimed at purifying, characterizing, elucidating the mode of action, and enhancing the production of salivaricin from Lactobacillus salivarius of human gut origin was conducted. Results: Salivaricin mmaye1 is a novel bacteriocin purified from L. salivarius isolated from human feces. It is potent at micromolar concentrations and has a molecular weight of 1221.074 Da as determined by MALDI-TOF mass spectrometry. It has a broad spectrum of antibacterial activity. Salivaricin mmaye1 showed high thermal and chemical stability and moderate pH stability. The proteinaceous nature of salivaricin mmaye1 was revealed by the complete loss of activity after treatment with pepsin, trypsin, α-chymotrypsin, protease, and proteinase. Salivaricin mmaye1 is cell wall associated, and adsorption–desorption of the bacteriocin from the cell wall of the producer by pH modification proved successful. It exhibited a bactericidal mode of action mediated by pore formation. Its biosynthesis is regulated by a quorum sensing mechanism. Enhanced production of salivaricin mmaye1 was achieved in a newly developed growth medium. Conclusions: A novel, cell wall adhering, highly potent bacteriocin with a broad spectrum of inhibitory activity, membrane-permeabilizing ability, and enhanced production in a newly constituted medium has been isolated. It has a quorum sensing regulatory system and possesses interesting physicochemical characteristics favoring its future use in food biopreservation. These findings pave the way for future evaluation of its medical and food applications.

Item Type: Article
Funders: High Impact Research-Malaysian Ministry of Higher Education grant designated as UM.C/625/1/HIR/MOHE/SC/08 with account number F000008-21001, Postgraduate Research Grant with project number PG353-2016A under the Principal Investigator Koshy Philip
Uncontrolled Keywords: Antibiotic resistance; Bactericidal; Bacteriocin production; Bacteriocin; Cell wall-associated bacteriocin; Food spoilage bacteria; Human feces; Lactobacillus salivarius; Pore formation; Quorum sensing; Salivaricin
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 18 Feb 2019 08:53
Last Modified: 18 Feb 2019 08:53
URI: http://eprints.um.edu.my/id/eprint/20385

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