Enrichment of PHA-accumulators for sustainable PHA production from crude glycerol

Mohamad Fauzi, Ainil Hawa and Chua, Adeline Seak May and Yoon, Li Wan and Nittami, Tadashi and Yeoh, Hak Koon (2019) Enrichment of PHA-accumulators for sustainable PHA production from crude glycerol. Process Safety and Environmental Protection, 122. pp. 200-208. ISSN 0957-5820, DOI https://doi.org/10.1016/j.psep.2018.12.002.

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Official URL: https://doi.org/10.1016/j.psep.2018.12.002


Polyhydroxyalkanoates (PHAs) as biodegradable polymers produced by microorganisms with thermo-plastic properties are of high interest. In this study, PHA-accumulators were cultivated using crude glycerol and activated sludge in a sequencing batch reactor (SBR) with the aerobic dynamic feeding (ADF) strategy. This study aimed to enrich PHA-accumulators by evaluating the stability of the cultivation reactor followed by the effect of organic loading rate (OLR). The cultivation reactor was stable while maintaining a steady feast/famine (F/F) ratio. The increase of OLR from 360 mgC/(L·d) to 1000 mgC/(L·d) led to the increment of biomass concentration from less than 0.7 g/L to 2 g/L. The enriched mixed culture produced a maximum PHA content of 80 wt% in biomass dry weight with a production yield of 0.7 mg C PHA/mg C. The mixed culture were found to accumulate both 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3 HV) monomers at a HB:HV molar ratio of 60:40. Based on fluorescence in situ hybridization (FISH) analysis, Alphaproteobacteria and Betaproteobacteria were the most dominant species during peak PHA production. This method provides an option for resource recovery operation in converting waste to value-added products.

Item Type: Article
Funders: University of Malaya Postgraduate Research Grant (PG268-2015B), University of Malaya Research Grant (RP002C-13AET)
Uncontrolled Keywords: Aerobic dynamic feeding; Sequencing batch reactor; Crude glycerol; Mixed microbial culture; Organic loading rate; Polyhydroxyalkanoates (PHA)
Subjects: T Technology > TP Chemical technology
Divisions: Faculty of Engineering
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 17 Jan 2019 02:51
Last Modified: 17 Jan 2019 02:51
URI: http://eprints.um.edu.my/id/eprint/20031

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