Dengue Patients Exhibit Higher Levels of PrM and E Antibodies Than Their Asymptomatic Counterparts

Yeo, A.S.L. and Rathakrishnan, A. and Wang, S.M. and Ponnampalavanar, S. and Manikam, R. and Sathar, J. and Kumari Natkunam, S. and Sekaran, S.D. (2015) Dengue Patients Exhibit Higher Levels of PrM and E Antibodies Than Their Asymptomatic Counterparts. BioMed Research International, 2015. pp. 1-10. ISSN 2314-6133, DOI

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Dengue virus infection is a common tropical disease which often occurs without being detected. These asymptomatic cases provide information in relation to the manifestation of immunological aspects. In this study, we developed an ELISA method to compare neutralizing effects of dengue prM and E antibodies between dengue patients and their asymptomatic household members. Recombinant D2 premembrane (prM) was constructed, cloned, and tested for antigenicity. The recombinant protein was purified and tested with controls by using an indirect ELISA method. Positive dengue serum samples with their asymptomatic pair were then carried out onto the developed ELISA. In addition, commercially available recombinant envelope (E) protein was used to develop an ELISA which was tested with the same set of serum samples in the prM ELISA. Asymptomatic individuals showed preexisting heterotypic neutralizing antibodies. The recombinant prM was antigenically reactive in the developed ELISA. Dengue patients had higher prM and E antibodies compared to their household members. Our study highlights the neutralizing antibodies levels with respect to dengue prM and E between dengue patients and asymptomatic individuals.

Item Type: Article
Funders: High Impact Research Grant UM-MOHE UM.C/625/1/HIR/MOHE/H-20001-00-E000053, UM Postgraduate Research Fund (PV039/2012A)
Uncontrolled Keywords: Antibodies, Neutralizing; Antibodies, Viral; Antigens, ViralBase Sequence; Blotting, Western; Dengue; Enzyme-Linked Immunosorbent Assay; Family Characteristics; Humans; Molecular Sequence Data; Polymerase Chain Reaction; Recombinant Proteins; Restriction Mapping; Sequence Analysis, DNA
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 21 Sep 2018 06:19
Last Modified: 21 Sep 2018 06:19

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