Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling

Al-Idrus, A. and Carpentier, S.C. and Ahmad, M.T. and Panis, B. and Mohamed, Z. (2017) Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling. PLoS ONE, 12 (6). e0178438. ISSN 1932-6203, DOI https://doi.org/10.1371/journal.pone.0178438.

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Official URL: http://dx.doi.org/10.1371/journal.pone.0178438


With a diverse host range, Meloidogyne incognita (root-knot nematode) is listed as one of the most economically important obligate parasites of agriculture. This nematode species establishes permanent feeding sites in plant root systems soon after infestation. A compatible host-nematode interaction triggers a cascade of morphological and physiological process disruptions of the host, leading to pathogenesis. Such disruption is reflected by altered gene expression in affected cells, detectable using molecular approaches. We employed a high-throughput proteomics approach to elucidate the events involved in a compatible banana-M. incognita interaction. This study serves as the first crucial step in developing natural banana resistance for the purpose of biological-based nematode management programme. We successfully profiled 114 Grand naine root proteins involved in the interaction with M. incognita at the 30th- and 60th- day after inoculation (dai). The abundance of proteins involved in fundamental biological processes, cellular component organisation and stress responses were significantly altered in inoculated root samples. In addition, the abundance of proteins in pathways associated with defence and giant cell maintenance in plants such as phenylpropanoid biosynthesis, glycolysis and citrate cycle were also implicated by the infestation.

Item Type: Article
Funders: Ministry of Science, Technology and Innovation Malaysia, under Top-down Grant [number 53-02-03-1069, 2010], University of Malaya, Kuala Lumpur, Malaysia under Postgraduate research Grant [number PG194-2014B, 2014], and UMRG grant [number RP005E-13BIO, 2013], University of Malaya: Flagship programme [number FL002-2010, 2010]
Uncontrolled Keywords: Animals; Chromatography, Liquid; High-Throughput Screening Assays; Musa; Proteome; Tandem Mass Spectrometry; Tylenchoidea
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 18 Sep 2018 04:22
Last Modified: 18 Sep 2018 04:22
URI: http://eprints.um.edu.my/id/eprint/19245

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